EMPIAR-11129 - Cryo-EM of ADP-Pi-F-actin [multiple data sets in TIFF and MRCS formats]

EMPIAR-11129

Cryo-EM of ADP-Pi-F-actin [multiple data sets in TIFF and MRCS formats]

Publication:

Bending forces and nucleotide state jointly regulate F-actin structure

Reynolds MJ, Hachicho C, Carl AG, Gong R, Alushin GM

Nature 611 (2022) 380-386

PMID: 36289330

DOI: 10.1038/s41586-022-05366-w

Related EMDB entry:

EMD-27117 EMD-27115

Deposited:

2022-07-29

Released:

2022-09-13

Last modified:

2022-11-11

Imageset size:

2.53 TB

Imageset DOI:

10.6019/EMPIAR-11129

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
picked particles - single frame - processed

Image Sets

1. Unaligned multi-frame micrographs of ADP-F-actin

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

4834

Image size:

(7420, 7676)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.515, 0.515)

Details:

This directory also contains motion-correction that was done on-the-fly. Re-running motion-correction locally is recommended.

Files:

Archives and downloads the selected files into an uncompressed zip file.

Depending on the number and size of files to be downloaded, this can take an enormous amount of time. Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.

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rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

2. Polished single-frame particles of bent ADP-Pi-F-actin segments

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

14991

Image size:

(512, 512)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.03, 1.03)

Details:

This stack of particles was generated from the final Refine3D of the asymmetric, bent ADP-Pi-F-actin at EMD-27117 and PDB: 8D16 using the relion_stack_create command.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Polished single-frame particles of helical ADP-Pi-F-actin segments, for high-resolution

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

374778

Image size:

(512, 512)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.03, 1.03)

Details:

This set of particles was used for the final Refine3D of the helically-symmetric ADP-Pi-F-actin at EMD-27115 and PDB: 8D14
The run_data.star file corresponds to the final Refine3D that was run using the polished particle stacks in the Polish directory.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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