EMPIAR-11013 - Cryo-EM images of equine apo-ferritin recorded as demonstration of MeasureIce software [626 multi-frame micrographs composed of 8 frames each in TIFF format]

EMPIAR-11013

Cryo-EM images of equine apo-ferritin recorded as demonstration of MeasureIce software [626 multi-frame micrographs composed of 8 frames each in TIFF format]

Publication:

MeasureIce: accessible on-the-fly measurement of ice thickness in cryo-electron microscopy

Brown HG, Hanssen E

Communications biology 5 (2022)

PMID: 35965271

DOI: 10.1038/s42003-022-03698-x

Related EMDB entry:

EMD-25619

Deposited:

2022-04-11

Released:

2022-09-06

Last modified:

2022-09-06

Imageset size:

136.79 GB

Imageset DOI:

10.6019/EMPIAR-11013

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multiframe micrographs of Equine Apoferritin

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

626

Image size:

(4096, 4096)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(0.637, 0.637)

Details:

Ignore the final frame of each fraction as this is the sum of the proceeding 7

Additional Notes:

Raw movies
7 fractions with final frame sum of proceeding, exclude frame #8 from data processing
LZW compressed TIFF
626 images
4096 x 4096 pixels
0.637 Angstrom pixel size
16-bit integer

Microscope parameters
300 kV
2.7 mm Cs
20 e/A^2 total dose

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Citations

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In situ structure determination of conformationally flexible targets using nextPYP.

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CryoPROS: Correcting misalignment caused by preferred orientation using AI-generated auxiliary particles.

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Few-shot learning for non-vitrified ice segmentation.

Vivas-Lago A, Castaño-Díez D. (2025)

Automated removal of corrupted tilts in cryo-electron tomography.

Majtner T, Turoňová B. (2025)

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Felix J, De Munck S, Bazan JF, Savvides SN. (2025)

Probabilistic single-particle cryo-EM ab initio 3D reconstruction in SIMPLE.

Van CTS, Reboul CF, Caesar JJE, Meana-Pañeda R, Lountos GT, Deme JC, Bryant OJ, Johnson S, Piczak CT, Valkov E, Lea SM, Elmlund H. (2025)

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