EMPIAR-10987 - Parallel cryo electron tomography (PACE-tomo) of 80S ribosomes in situ [multiple data sets in MRC format]

EMPIAR-10987

Parallel cryo electron tomography (PACE-tomo) of 80S ribosomes in situ [multiple data sets in MRC format]

Publication:

Parallel cryo electron tomography on in situ lamellae

Eisenstein F, Yanagisawa H, Kashihara H, Kikkawa M, Tsukita S, Danev R

bioRxiv (2022)

DOI: 10.1101/2022.04.07.487557

Related EMDB entry:

EMD-33118

Deposited:

2022-03-17

Released:

2022-06-14

Last modified:

2023-02-01

Imageset size:

132.30 GB

Imageset DOI:

10.6019/EMPIAR-10987

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
tilt series

Image Sets

1. Gain corrected movie frames for all tilt series

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

861

Image size:

(4092, 5760)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.64, 1.64)

Details:

considerable intra-frame motion present

Files:

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Depending on the number and size of files to be downloaded, this can take an enormous amount of time. Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.

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rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

2. Tilt series and meta data

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

Image size:

(4092, 5760)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(1.64, 1.64)

Details:

Tilt series sorted by tilt angle, mdoc files and dose files usable in IMOD

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

CryoTRANS: predicting high-resolution maps of rare conformations from self-supervised trajectories in cryo-EM.

Fan X, Zhang Q, Zhang H, Zhu J, Ju L, Shi Z, Hu M, Bao C. (2024)

See all citations

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