EMPIAR-10929 - CryoET of presequence protease single particle [multiple data sets in TIFF and MRC formats]

EMPIAR-10929

CryoET of presequence protease single particle [multiple data sets in TIFF and MRC formats]

Publication:

Structural basis for the mechanisms of human presequence protease conformational switch and substrate recognition

Liang WG, Wijaya J, Wei H, Noble AJ, Mancl JM, Mo S, Lee D, Lin King JV, Pan M, Liu C, Koehler CM, Zhao M, Potter CS, Carragher B, Li S, Tang WJ

Nature communications 13 (2022)

PMID: 35383169

DOI: 10.1038/s41467-022-29322-4

Related EMDB entry:

EMD-25921

Deposited:

2022-01-14

Released:

2022-01-28

Last modified:

2022-10-17

Imageset size:

25.43 GB

Imageset DOI:

10.6019/EMPIAR-10929

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
reconstructed volumes

Image Sets

1. Unaligned K2 tilt image frame stacks

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

Image size:

(3710, 3838)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(1.331, 1.331)

Details:

Unaligned K2 tif tilt image frame stacks. Frame number increases from 8 at 0 degrees to 13 at high tilts to account for increasing thickness; dose increases from 3.66 to 5.94 e/A^2.
Gain references are in the references/ folder.

Files:

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2. Binned by 4 tomograms from all 7 tilt-series

Category:

reconstructed volumes

Image format:

MRC

No. of images or tilt series:

Image size:

(1200, 1130)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(5.324, 5.324)

Details:

Dose-compensated Tomo3D SIRT binned by 4 reconstructions from Appion-Protomo tilt-series alignments for each tilt-series in the provided frames directory.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

Structural basis for HIV-1 antagonism of host APOBEC3G via Cullin E3 ligase.

Ito F, Alvarez-Cabrera AL, Liu S, Yang H, Shiriaeva A, Zhou ZH, Chen XS. (2023)

The 2023 Nucleic Acids Research Database Issue and the online molecular biology database collection.

Rigden DJ, Fernández XM. (2023)

EMPIAR: the Electron Microscopy Public Image Archive.

Iudin A, Korir PK, Somasundharam S, Weyand S, Cattavitello C, Fonseca N, Salih O, Kleywegt GJ, Patwardhan A. (2023)

CLEMSite, a software for automated phenotypic screens using light microscopy and FIB-SEM.

Serra Lleti JM, Steyer AM, Schieber NL, Neumann B, Tischer C, Hilsenstein V, Holtstrom M, Unrau D, Kirmse R, Lucocq JM, Pepperkok R, Schwab Y. (2023)

Structure of a RecT/Redβ family recombinase in complex with a duplex intermediate of DNA annealing.

Caldwell BJ, Norris AS, Karbowski CF, Wiegand AM, Wysocki VH, Bell CE. (2022)

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