EMPIAR-10927 - Single particle cryo-EM dataset of Mus musculus mitochondrial complex I bound with an acetogenin inhibitor [1283 multi-frame micrographs composed of 50 frames each in MRC format]

EMPIAR-10927

Single particle cryo-EM dataset of Mus musculus mitochondrial complex I bound with an acetogenin inhibitor [1283 multi-frame micrographs composed of 50 frames each in MRC format]

Publication:

Cryo-electron microscopy reveals how acetogenins inhibit mitochondrial respiratory complex I

Grba DN, Blaza JN, Bridges HR, Agip AA, Yin Z, Murai M, Miyoshi H, Hirst J

The Journal of biological chemistry 298 (2022)

PMID: 35063503

DOI: 10.1016/j.jbc.2022.101602

Related EMDB entry:

EMD-13611

Deposited:

2021-12-07

Released:

2022-04-25

Last modified:

2022-04-25

Imageset size:

3.32 TB

Imageset DOI:

10.6019/EMPIAR-10927

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Single-particle cryo-EM dataset of Mus musculus mitochondrial complex I co-purified with the inhibitor acetogenin

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

1283

Image size:

(3838, 3710)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.043, 1.043)

Details:

Raw, multi-frame micrographs (MRC files) are deposited. Micrographs were imaged using a Gatan K2 summit camera in counting mode and GIF Quantum energy filter (20 eV slit width) on an FEI 300 keV Titan Krios microscope with a 100 μm objective aperture. 10s exposures captured in 50 frames and a total dose of 50 electrons Å-2. A pixel size of 1.043 Å was used.

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