EMPIAR-10916 - Single particle cryo-EM dataset of sarkosyl-insoluble fraction from the frontal cortex of an individual with Alzheimer’s disease of amyloid-β 42 filaments [1762 multi-frame micrographs composed of 40 frames each in TIFF format]

EMPIAR-10916

Single particle cryo-EM dataset of sarkosyl-insoluble fraction from the frontal cortex of an individual with Alzheimer’s disease of amyloid-β 42 filaments [1762 multi-frame micrographs composed of 40 frames each in TIFF format]

Publication:

Cryo-EM structures of amyloid-β 42 filaments from human brains

Yang Y, Arseni D, Zhang WJ, Huang M, Lovestam SKA, Schweighauser M, Kotecha A, Murzin AG, Peak-Chew S, Macdonald J, Lavenir I, Garringer HJ, Gelpi E, Newell KL, Kovacs GG, Vidal R, Ghetti B, Falcon B, Scheres SHW, Goedert M

Science (New York, N.Y.) 375 (2022) 167-172

PMID: 35025654

DOI: 10.1126/science.abm7285

Related EMDB entry:

EMD-13800

Deposited:

2021-11-01

Released:

2022-01-25

Last modified:

2023-01-23

Imageset size:

531.91 GB

Imageset DOI:

10.6019/EMPIAR-10916

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame movies

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

1762

Image size:

(4096, 4096)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.727, 0.727)

Details:

The files were written as eer files by EPU and then converted to TIFF files by relion_convert_to_tiff. During this process, the pixel size in the header was lost. The real pixel size is 0.727 A/px. The dose rate is 1 e/A2/frame. Please use gain-reference.mrc as a gain reference without any rotation or flipping.
pick_coordinates contains start-end coordinates of the manually picked filaments.
Refine097_run_data.star contains STAR file(s) with the metadata of the final refined segments.

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Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

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