EMPIAR-10893 - Motion corrected micrographs - purified Dot/Icm T4SS particles [3590 micrographs in MRC format]

EMPIAR-10893

Motion corrected micrographs - purified Dot/Icm T4SS particles [3590 micrographs in MRC format]

Publication:

Structural analysis of the Legionella pneumophila Dot/Icm type IV secretion system core complex

Durie CL, Sheedlo MJ, Chung JM, Byrne BG, Su M, Knight T, Swanson M, Lacy DB, Ohi MD

eLife (2020)

PMID: 32876045

DOI: 10.7554/elife.59530

Related EMDB entry:

EMD-22070

Deposited:

2021-12-22

Released:

2022-01-12

Last modified:

2022-01-12

Imageset size:

190.43 GB

Imageset DOI:

10.6019/EMPIAR-10893

Experimental metadata:

Download xml json

Contains:

micrographs - single frame

Image Sets

1. Motion corrected micrographs Legionella pneumophila Dot/Icm T4SS

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

3590

Image size:

(3838, 3710)

Pixel type:

SIGNED 32 BIT INTEGER

Pixel spacing:

(1.64, 1.64)

Details:

The images of the T4SS complexes from wild-type cells were collected on the Thermo Fisher 300 kV Titan Krios with Gatan K2 Summit Direct Electron Detector (DED) camera having a nominal pixel size of 1.64 Å. Micrographs were acquired using Leginon software. The total exposure time was 16 s, and frames were recorded every 0.2 s, resulting in a total accumulated dose of 58.1 e- Å−2 using a defocus range of −1 to −3 μm. The video frames were aligned using Motioncor2.

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