EMPIAR-10888 - Motion corrected micrographs - purified SINV/EEEV particles and recombinant anti-EEEV Fab (EEEV-143) [3535 micrographs in MRC format]

EMPIAR-10888

Motion corrected micrographs - purified SINV/EEEV particles and recombinant anti-EEEV Fab (EEEV-143) [3535 micrographs in MRC format]

Publication:

Human Antibodies Protect against Aerosolized Eastern Equine Encephalitis Virus Infection

Williamson LE, Gilliland T, Yadav PK, Binshtein E, Bombardi R, Kose N, Nargi RS, Sutton RE, Durie CL, Armstrong E, Carnahan RH, Walker LM, Kim AS, Fox JM, Diamond MS, Ohi MD, Klimstra WB, Crowe JE

Cell 183 (2020) 1884-1.9e+26

PMID: 33301709

DOI: 10.1016/j.cell.2020.11.011

Related EMDB entry:

EMD-22188

Deposited:

2021-11-21

Released:

2022-01-14

Last modified:

2022-01-14

Imageset size:

187.46 GB

Imageset DOI:

10.6019/EMPIAR-10888

Experimental metadata:

Download xml json

Contains:

micrographs - single frame

Image Sets

1. Motion corrected micrographs Chimeric Eastern Equine Encephalitis Virus with Fab of EEEV-143 Antibody

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

3535

Image size:

(3838, 3710)

Pixel type:

SIGNED 32 BIT INTEGER

Pixel spacing:

(2.0, 2.0)

Details:

Images for SINV/EEEV:EEEV-143 Fab were collected on a Glacios electron microscope (Thermo Fisher Scientific) equipped with a K2 Summit Direct Electron Detector operated at 200 kV and having a nominal pixel size of 2.0 Å per pixel. Micrographs were acquired automatically using Leginon software. The total exposure time was 10 s and frames were recorded every 0.2 s. Defocus values ranged from 1.0 to 2.5 μm. The total accumulated does was ~25 e−/Å2. Deposited micrographs were motion corrected using MotionCor2.

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