EMPIAR-10841 - Raw movies and final particle stack for a dataset of bL17-limited E. coli ribosome assembly intermediates. [multiple data sets in MRCS and MRC formats]

EMPIAR-10841

Raw movies and final particle stack for a dataset of bL17-limited E. coli ribosome assembly intermediates. [multiple data sets in MRCS and MRC formats]

Publication:

Quantitative Mining of Compositional Heterogeneity in Cryo-EM Datasets of Ribosome Assembly Intermediates

Rabuck-Gibbons JN, Lyumkis D, Williamson JR

bioRxiv (2021)

DOI: 10.1101/2021.06.23.449614

Related EMDB entry:

EMD-24509 EMD-24508 EMD-24499 EMD-24520 EMD-24517 EMD-24491 EMD-24510 EMD-24492 EMD-24515 EMD-24658 EMD-24659 EMD-24660 EMD-24634 EMD-24662 EMD-24661 EMD-24633 EMD-24631 EMD-24627 EMD-24573 EMD-24626 EMD-24574 EMD-24555 EMD-24546 EMD-24538 EMD-24539 EMD-24550 EMD-24543 EMD-24521 EMD-24527 EMD-24558 EMD-24673 EMD-24671 EMD-24669 EMD-24571 EMD-24567 EMD-24568 EMD-24562 EMD-24561 EMD-24565 EMD-24563 EMD-24564 EMD-24559

Deposited:

2021-10-20

Released:

2022-01-12

Last modified:

2022-01-12

Imageset size:

1.26 TB

Imageset DOI:

10.6019/EMPIAR-10841

Experimental metadata:

Download xml json

Contains:

picked particles - single frame - processed
micrographs - multiframe

Image Sets

1. Particle stack file of L17-depleted ribosomal subunits used for 3D classification

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

123804

Image size:

(160, 160)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(2.64, 2.64)

Details:

Final particle stack for the dataset of bL17-limited ribosome assembly intermediates. Fluence is 35 e-/A^2. All information about the particles is provided in the L17all.star file.

Files:

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2. Movies of L17-depleted ribosomal subunits used for 3D classification

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

481

Image size:

(7420, 7676)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.66, 0.66)

Details:

---

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

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