EMPIAR-10823 - Cryo electron micrographs of Pol delta-PCNA-DNA-FEN1 sample [5073 multi-frame micrographs composed of 45 frames each in TIFF format]

EMPIAR-10823

Cryo electron micrographs of Pol delta-PCNA-DNA-FEN1 sample [5073 multi-frame micrographs composed of 45 frames each in TIFF format]

Publication:

Structure of the processive human Pol δ holoenzyme

Lancey C, Tehseen M, Raducanu VS, Rashid F, Merino N, Ragan TJ, Savva CG, Zaher MS, Shirbini A, Blanco FJ, Hamdan SM, De Biasio A

Nature communications 11 (2020)

PMID: 32111820

DOI: 10.1038/s41467-020-14898-6

Related EMDB entry:

EMD-10539 EMD-10540

Deposited:

2021-08-19

Released:

2021-10-22

Last modified:

2021-10-22

Imageset size:

1.58 TB

Imageset DOI:

10.6019/EMPIAR-10823

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Cryo-electron micrographs of Pol delta-FEN1 toolbelt

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

5073

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.435, 0.435)

Details:

Images taken using a FEI Titan Krios G3 with Gatan K3 camera in super resolution mode (binning 1). Defocus range -2.3 to -1.1. Total dose = 43.6 e-/Å^2. Dataset contains 743 corrupted or partially corrupted movies, as file names suggest - these became corrupted after the deposited EMDB maps were generated. The given pixel size accounts for super resolution binning 1 - the physical pixel size was 0.87 Å/pix.

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