EMPIAR-10795 - A microtubule RELION-based pipeline for cryo-EM image processing [1128 multi-frame micrographs composed of 64 frames each in MRC format]

EMPIAR-10795

A microtubule RELION-based pipeline for cryo-EM image processing [1128 multi-frame micrographs composed of 64 frames each in MRC format]

Publication:

A microtubule RELION-based pipeline for cryo-EM image processing

Cook AD, Manka SW, Wang S, Moores CA, Atherton J

Journal of Structural Biology (2019)

PMID: 31610239

DOI: 10.1016/j.jsb.2019.10.004

Related EMDB entry:

EMD-4643

Deposited:

2019-07-24

Released:

2021-09-24

Last modified:

2022-02-04

Imageset size:

1.81 TB

Imageset DOI:

10.6019/EMPIAR-10795

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Movies of microtubules decorated with CAMSAP1-CKK domain

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

1128

Image size:

(3710, 3838)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(None, None)

Details:

64 frames over 16 seconds.
42 electron per squared Angstrom total dose over the whole movie.
Pixel size 1.39 Angstrom/Pixel.
Camera and microscope: K2 summit in counting mode on a Technai Polara at 300kV.

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Citations

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Kim K, Li H, Clarke OB. (2025)

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CryoEMNet driven symmetry-aware molecular reconstruction through deep learning enhanced electron microscopy.

Arora S, Pan SH, Kumar S, Singh SK, Chauhan U, Alhalabi W, Arya V, Alsulami BS, Hsu CH, Chui KT, Gupta BB. (2025)

PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

Costa-Gomes B, Greer J, Juraschko N, Parkhurst J, Mirecka J, Famili M, Rangel-Smith C, Strickson O, Lowe A, Basham M, Burnley T. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

Laser flash melting cryo-EM samples to overcome preferred orientation.

Straub MS, Harder OF, Mowry NJ, Barrass SV, Hruby J, Drabbels M, Lorenz UJ. (2025)

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Duraisamy AK, Fonseca N, Kleywegt GJ, Patwardhan A, Morris KL. (2025)

Global conformations of <i>Pichia pastoris</i> complex I are distinguished by the binding of a unique interdomain bridging subunit.

Lee CS, Grba DN, Wright JJ, Ivanov BS, Hirst J. (2025)

Overcoming Preferred Orientation in Cryo-EM With Ultrasonic Excitation During Vitrification

Williams HM, Curtis WA, Haubner M, Hruby J, Drabbels M, Lorenz UJ. (2025)

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