EMPIAR-10771 - Cryo-electron tomography of rod outer segments in ABCA4 knockout mice acquired with Volta phase plate in focus [2 reconstructed volumes in MRC format]

EMPIAR-10771

Cryo-electron tomography of rod outer segments in ABCA4 knockout mice acquired with Volta phase plate in focus [2 reconstructed volumes in MRC format]

Publication:

Determinants shaping the nanoscale architecture of the mouse rod outer segment

Pöge M, Mahamid J, Imanishi SS, Plitzko JM, Palczewski K, Baumeister W

eLife 10 (2021)

PMID: 34931611

DOI: 10.7554/elife.72817

Related EMDB entry:

EMD-13324

Deposited:

2021-07-21

Released:

2022-01-07

Last modified:

2022-01-07

Imageset size:

2.98 GB

Imageset DOI:

10.6019/EMPIAR-10771

Experimental metadata:

Download xml json

Contains:

reconstructed volumes

Image Sets

1. Tomograms of rod outer segments in ABCA4 knockout mice acquired with Volta phase plate

Category:

reconstructed volumes

Image format:

MRC

No. of images or tilt series:

Image size:

(928, 928)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(10.48, 10.48)

Details:

4x binned and dose filtered tomograms with dimensions 928x928x464. Tilt series were acquired conventionally with defocus. Projection images were recorded between -60° and 60° with an increment of 2°. The 400 frames are distributed over all projections. The total exposure per tilt-series was ~ 100e-/A^2.

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Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

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Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

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