EMPIAR-10740 - Cryo-EM of Mechanosensitive Ion Channel Flycatcher1 in GDN [multiple data sets in MRC format]

EMPIAR-10740

Cryo-EM of Mechanosensitive Ion Channel Flycatcher1 in GDN [multiple data sets in MRC format]

Publication:

Structural insights into the Venus flytrap mechanosensitive ion channel Flycatcher1

Jojoa-Cruz S, Saotome K, Tsui CCA, Lee WH, Sansom MSP, Murthy SE, Patapoutian A, Ward AB

Nature communications 13 (2022)

PMID: 35165281

DOI: 10.1038/s41467-022-28511-5

Related EMDB entry:

EMD-24186 EMD-24189 EMD-24188 EMD-24187

Deposited:

2021-06-07

Released:

2022-02-25

Last modified:

2022-02-25

Imageset size:

876.94 GB

Imageset DOI:

10.6019/EMPIAR-10740

Experimental metadata:

Download xml json

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

MRC movies (*frames.mrc) in this entry were compressed by bzip2 (pbzip2)
by EMPIAR Japan to save space and bandwidth.

Some programs (e.g. RELION >= 4.0.1 and CryoSPARC) can process compressed movies without explicit
decompression. For other programs, movies have to be decompressed beforehand. In this case,
you might want to convert decompressed MRC movies into TIFF to save your local storage.

Original size:   6,100,741,504 kilobytes
Compressed size:   919,375,564 kilobytes

Contains:

micrographs - multiframe
micrographs - single frame

Image Sets

1. Unaligned multi-frame micrographs of FLYC1 in GDN, dataset 1

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

2648

Image size:

(3710, 3838)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(1.03, 1.03)

Details:

particles.star file corresponds to only particles from this dataset (dataset 1) and not dataset 2. Only particles from dataset 1 were used for bayesian polishing and final refinements to obtain the final C1 volume (before symmetry expansion).
Description_FLYC1datasets_v2.txt file contains further information about data collection.

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2. Unaligned multi-frame micrographs of FLYC1 in GDN, dataset 2

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

2648

Image size:

(3710, 3838)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(1.03, 1.03)

Details:

---

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Gain references for dataset 1 and dataset 2

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

Image size:

(3710, 3838)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.03, 1.03)

Details:

Gain references for each dataset:
-Gain reference for dataset 1: 19may24k_ref.mrc
-Gain reference for dataset 2: 19jul28d_ref.mrc

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

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