EMPIAR-10725 - Single particle cryo EM dataset of SARS-CoV-2 Spike protein with D614G substitution [10051 multi-frame micrographs composed of 32 frames each in MRC format]

EMPIAR-10725

Single particle cryo EM dataset of SARS-CoV-2 Spike protein with D614G substitution [10051 multi-frame micrographs composed of 32 frames each in MRC format]

Publication:

The effect of the D614G substitution on the structure of the spike glycoprotein of SARS-CoV-2

Benton DJ, Wrobel AG, Roustan C, Borg A, Xu P, Martin SR, Rosenthal PB, Skehel JJ, Gamblin SJ

Proceedings of the National Academy of Sciences of the United States of America 118 (2021)

PMID: 33579792

DOI: 10.1073/pnas.2022586118

Related EMDB entry:

EMD-12231 EMD-12230 EMD-12229

Deposited:

2021-05-18

Released:

2021-06-11

Last modified:

2021-06-11

Imageset size:

4.51 TB

Imageset DOI:

10.6019/EMPIAR-10725

Experimental metadata:

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Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

MRC movies (*Fractions.mrc) in this entry were compressed by bzip2 (pbzip2)
by EMPIAR Japan to save space and bandwidth.

Some programs (e.g. RELION >= 4.0.1 and CryoSPARC) can process compressed movies without explicit
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Original size:   10,539,247,427 kilobytes
Compressed size:  4,839,062,181 kilobytes

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of SARS-CoV-2 Spike with D614G substitution

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

10051

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(0.85, 0.85)

Details:

Uncorrected movies of D614G SARS-CoV-2 spike used to calculate closed, 1 erect RBD and 2 erect RBD forms of this mutant spike. Images were taken on FEI Krios G2 equipped with a Falcon 3 operating in electron counting mode. Images were 40s exposures fractionated into 32 frame gain corrected mrc files, with an accumulated dose of 36.8 e/Å^2. Images were collected with nominal defocus of 1 - 3 µm applied.

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Citations

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Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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