EMPIAR-10716 - Cryo electron microscopy of single particles of the Haliangium ochraceum Encapsulin:encapsulated ferritin encapsulin nano compartment [8109 multi-frame micrographs composed of 40 frames each in TIFF format]

EMPIAR-10716

Cryo electron microscopy of single particles of the Haliangium ochraceum Encapsulin:encapsulated ferritin encapsulin nano compartment [8109 multi-frame micrographs composed of 40 frames each in TIFF format]

Publication:

Pore dynamics and asymmetric cargo loading in an encapsulin nanocompartment

Ross J, McIver Z, Lambert T, Piergentili C, Bird JE, Gallagher KJ, Cruickshank FL, James P, Zarazúa-Arvizu E, Horsfall LE, Waldron KJ, Wilson MD, Mackay CL, Baslé A, Clarke DJ, Marles-Wright J

Science advances 8 (2022)

PMID: 35080974

DOI: 10.1126/sciadv.abj4461

Related EMDB entry:

EMD-12853 EMD-12864 EMD-12859 EMD-12873

Deposited:

2021-05-11

Released:

2022-03-01

Last modified:

2022-03-01

Imageset size:

1.83 TB

Imageset DOI:

10.6019/EMPIAR-10716

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Multi-frame micrographs and particle sets for Haliangium ochraceum encapsulin:encapsulated ferritin reconstruction

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

8109

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.326, 0.326)

Details:

Raw micrographs provided. Particles sets used for full I1 3D refinement provides with subsets for C1 and the symmetry expanded focused refinements.

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Citations

High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles

Kim K, Li H, Clarke OB. (2025)

CLEM-Reg: an automated point cloud-based registration algorithm for volume correlative light and electron microscopy.

Krentzel D, Elphick M, Domart MC, Peddie CJ, Laine RF, Shand C, Henriques R, Collinson LM, Jones ML. (2025)

CryoEMNet driven symmetry-aware molecular reconstruction through deep learning enhanced electron microscopy.

Arora S, Pan SH, Kumar S, Singh SK, Chauhan U, Alhalabi W, Arya V, Alsulami BS, Hsu CH, Chui KT, Gupta BB. (2025)

PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

Costa-Gomes B, Greer J, Juraschko N, Parkhurst J, Mirecka J, Famili M, Rangel-Smith C, Strickson O, Lowe A, Basham M, Burnley T. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

Laser flash melting cryo-EM samples to overcome preferred orientation.

Straub MS, Harder OF, Mowry NJ, Barrass SV, Hruby J, Drabbels M, Lorenz UJ. (2025)

In situ structure determination of conformationally flexible targets using nextPYP.

Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)

EMICSS: added-value annotations for EMDB entries.

Duraisamy AK, Fonseca N, Kleywegt GJ, Patwardhan A, Morris KL. (2025)

Global conformations of <i>Pichia pastoris</i> complex I are distinguished by the binding of a unique interdomain bridging subunit.

Lee CS, Grba DN, Wright JJ, Ivanov BS, Hirst J. (2025)

Overcoming Preferred Orientation in Cryo-EM With Ultrasonic Excitation During Vitrification

Williams HM, Curtis WA, Haubner M, Hruby J, Drabbels M, Lorenz UJ. (2025)

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