EMPIAR-10632 - Cryo electron microscopy of alpha-synuclein H50Q fibrils [3577 multi-frame micrographs composed of 30 frames each in MRC format]

EMPIAR-10632

Cryo electron microscopy of alpha-synuclein H50Q fibrils [3577 multi-frame micrographs composed of 30 frames each in MRC format]

Publication:

Structures of fibrils formed by α-synuclein hereditary disease mutant H50Q reveal new polymorphs

Boyer DR, Li B, Sun C, Fan W, Sawaya MR, Jiang L, Eisenberg DS

Nature structural & molecular biology 26 (2019) 1044-1052

PMID: 31695184

DOI: 10.1038/s41594-019-0322-y

Related EMDB entry:

EMD-20328

Deposited:

2021-01-26

Released:

2021-03-03

Last modified:

2021-03-03

Imageset size:

639.19 GB

Imageset DOI:

10.6019/EMPIAR-10632

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned K2 movies of alpha-synuclein H50Q fibrils

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

3577

Image size:

(3838, 3710)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(1.07, 1.07)

Details:

Movies are unaligned. 30 frames. Gain image does not need to be flipped. Grouped movies into two groups, first 2533 images had average dose of ~4.8 e/A^2/s, last 1024 images had dose of 3.2 e/A^2/s.

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Citations

High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles

Kim K, Li H, Clarke OB. (2025)

CLEM-Reg: an automated point cloud-based registration algorithm for volume correlative light and electron microscopy.

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CryoEMNet driven symmetry-aware molecular reconstruction through deep learning enhanced electron microscopy.

Arora S, Pan SH, Kumar S, Singh SK, Chauhan U, Alhalabi W, Arya V, Alsulami BS, Hsu CH, Chui KT, Gupta BB. (2025)

PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

Costa-Gomes B, Greer J, Juraschko N, Parkhurst J, Mirecka J, Famili M, Rangel-Smith C, Strickson O, Lowe A, Basham M, Burnley T. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

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Straub MS, Harder OF, Mowry NJ, Barrass SV, Hruby J, Drabbels M, Lorenz UJ. (2025)

In situ structure determination of conformationally flexible targets using nextPYP.

Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)

EMICSS: added-value annotations for EMDB entries.

Duraisamy AK, Fonseca N, Kleywegt GJ, Patwardhan A, Morris KL. (2025)

Global conformations of <i>Pichia pastoris</i> complex I are distinguished by the binding of a unique interdomain bridging subunit.

Lee CS, Grba DN, Wright JJ, Ivanov BS, Hirst J. (2025)

Overcoming Preferred Orientation in Cryo-EM With Ultrasonic Excitation During Vitrification

Williams HM, Curtis WA, Haubner M, Hruby J, Drabbels M, Lorenz UJ. (2025)

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