Unaligned frames are not corrected for detector’s gain and defects. Ten frames were saved per each tilt image. The exposure dose per tilt image was 3.0 e/Å^2. Calibrated pixel size 1.379 Å. Movies are named as ts_[tilt stack ID]_[sequntial movie number]_[tilt angle].tif. For-gain reference and defect correction, use data/correction/CountRef.dm4 and data/correction/defects.txt
Defocus estimation was performed using IMOD ctfplotter on non-dose filtered aligned stacks (data/aligned_stacks).
ETOMO-generated tilt alignment using 10 nm gold fiducials tracking
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Corrected, aligned and order-sorted tilt series. The tilt-series were generated from raw frames (data/rawframes) by applying gain reference and defect corrections, motion correction and Fourier cropping to physical pixel size. No dose-filtering was applied.
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