EMPIAR-10631 - Cryo-electron tomography of the fungal membrane-assembled retromer:Grd19 coat containing Kex2 cargo motif [multiple data sets in TIFF and MRC formats]

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EMPIAR-10631

Cryo-electron tomography of the fungal membrane-assembled retromer:Grd19 coat containing Kex2 cargo motif [multiple data sets in TIFF and MRC formats]

Publication:

Architecture and mechanism of metazoan retromer:SNX3 tubular coat assembly

Leneva N, Kovtun O, Morado DR, Briggs JAG, Owen DJ

Science advances 7 (2021)

PMID: 33762348

DOI: 10.1126/sciadv.abf8598

Related EMDB entry:

EMD-12225 EMD-12224 EMD-12223

Deposited:

2020-12-22

Released:

2021-07-09

Last modified:

2021-07-09

Imageset size:

347.68 GB

Imageset DOI:

10.6019/EMPIAR-10631

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
tilt series

Image Sets

1. Raw image frames for the fungal retromer:Grd19 coat assembled on the Kex2 cargo-containing membranes

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

Image size:

(3710, 3838)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(1.379, 1.379)

Details:

Unaligned frames are not corrected for detector’s gain and defects. Ten frames were saved per each tilt image. The exposure dose per tilt image was 3.0 e/Å^2. Calibrated pixel size 1.379 Å. Movies are named as ts_[tilt stack ID]_[sequntial movie number]_[tilt angle].tif. For-gain reference and defect correction, use data/correction/CountRef.dm4 and data/correction/defects.txt

Defocus estimation was performed using IMOD ctfplotter on non-dose filtered aligned stacks (data/aligned_stacks).

ETOMO-generated tilt alignment using 10 nm gold fiducials tracking

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2. Corrected, aligned and order-sorted tilt series for the membrane-reconstituted fungal retromer:Grd19 complex in the presence of cargo-signal containing C-portion of the Kex2 cargo.

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

Image size:

(3710, 3838)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(1.379, 1.379)

Details:

Corrected, aligned and order-sorted tilt series. The tilt-series were generated from raw frames (data/rawframes) by applying gain reference and defect corrections, motion correction and Fourier cropping to physical pixel size. No dose-filtering was applied.

Files:

This entry cannot be downloaded from PDBj.

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3. Corrected, aligned, dose-filtered and order-sorted tilt series for the membrane-reconstituted fungal retromer:Grd19 complex in the presence of cargo-signal containing C-portion of the Kex2 cargo.

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

Image size:

(3710, 3710)

Pixel type:

SIGNED 32 BIT INTEGER

Pixel spacing:

(1.379, 1.379)

Details:

Dose-filtered aligned stacks

Files:

This entry cannot be downloaded from PDBj.

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