EMPIAR-10606 - Movies of a heptameric light-harvesting 2 complex from the marine purple bacterium Mch. purpuratum [9543 multi-frame micrographs composed of 42 frames each in MRCS format]

EMPIAR-10606

Movies of a heptameric light-harvesting 2 complex from the marine purple bacterium Mch. purpuratum [9543 multi-frame micrographs composed of 42 frames each in MRCS format]

Publication:

The 2.4 Å cryo-EM structure of a heptameric light-harvesting 2 complex reveals two carotenoid energy transfer pathways

Gardiner AT, Naydenova K, Castro-Hartmann P, Nguyen-Phan TC, Russo CJ, Sader K, Hunter CN, Cogdell RJ, Qian P

Science advances 7 (2021)

PMID: 33579696

DOI: 10.1126/sciadv.abe4650

Related EMDB entry:

EMD-11516

Deposited:

2020-12-23

Released:

2021-03-05

Last modified:

2021-03-05

Imageset size:

6.05 TB

Imageset DOI:

10.6019/EMPIAR-10606

Experimental metadata:

Download xml json

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

MRC movies (*.mrcs) in this entry were compressed by bzip2 (pbzip2)
by EMPIAR Japan to save space and bandwidth.

Some programs (e.g. RELION >= 4.0.1 and CryoSPARC) can process compressed movies without explicit
decompression. For other programs, movies have to be decompressed beforehand. In this case,
you might want to convert decompressed MRC movies into TIFF to save your local storage.

Original size:   13,133,620,555 kilobytes
Compressed size:  6,492,292,402 kilobytes

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of purple bacterial LH2 on HexAuFoil supports

Category:

micrographs - multiframe

Image format:

MRCS

No. of images or tilt series:

9543

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(0.646, 0.646)

Details:

These are the non-aligned, gain-corrected movie stacks.

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Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

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EMPIAR: the Electron Microscopy Public Image Archive.

Iudin A, Korir PK, Somasundharam S, Weyand S, Cattavitello C, Fonseca N, Salih O, Kleywegt GJ, Patwardhan A. (2023)

CLEMSite, a software for automated phenotypic screens using light microscopy and FIB-SEM.

Serra Lleti JM, Steyer AM, Schieber NL, Neumann B, Tischer C, Hilsenstein V, Holtstrom M, Unrau D, Kirmse R, Lucocq JM, Pepperkok R, Schwab Y. (2023)

Structure of a RecT/Redβ family recombinase in complex with a duplex intermediate of DNA annealing.

Caldwell BJ, Norris AS, Karbowski CF, Wiegand AM, Wysocki VH, Bell CE. (2022)

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