EMPIAR-10588 - Cryo-EM reconstruction of heavy chain mouse apoferritin, 8 datasets testing dose rate and correlative double sampling mode for the Gatan K3 direct electron detector. [1143 multi-frame micrographs composed of 120 frames in LZW compressed TIFF format [multiple data sets in TIFF format]

EMPIAR-10588

Cryo-EM reconstruction of heavy chain mouse apoferritin, 8 datasets testing dose rate and correlative double sampling mode for the Gatan K3 direct electron detector. [1143 multi-frame micrographs composed of 120 frames in LZW compressed TIFF format [multiple data sets in TIFF format]

Publication:

Practical considerations for using K3 cameras in CDS mode for high-resolution and high-throughput single particle cryo-EM

Sun M, Azumaya CM, Tse E, Bulkley DP, Harrington MB, Gilbert G, Frost A, Southworth D, Verba KA, Cheng Y, Agard DA

Journal of structural biology 213 (2021)

PMID: 33984504

DOI: 10.1016/j.jsb.2021.107745

Related EMDB entry:

EMD-22972

Deposited:

2020-11-10

Released:

2021-11-26

Last modified:

2021-11-26

Imageset size:

8.48 TB

Imageset DOI:

10.6019/EMPIAR-10588

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. apoferritin multi-frame raw micrographs non gain normalized frames in LZW compressed TIFF format collected at 8 e/p/s in CDS mode from Gatan K3

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

1143

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.417, 0.417)

Details:

1143 multi-frame raw micrographs composed of 120 frames each in LZW compressed TIFF format, along with separate gain reference for the 8 e-/pix/s dose rate dataset at 105kx nominal magnification collected in CDS mode of Gatan K3.

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2. apoferritin multi-frame raw micrographs non gain normalized frames in LZW compressed TIFF format collected at 8 e/p/s in standard mode from Gatan K3

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

1143

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.417, 0.417)

Details:

Files:

Archives and downloads the selected files into an uncompressed zip file.

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Download a list of selected files.

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3. apoferritin multi-frame raw micrographs non gain normalized frames in LZW compressed TIFF format collected at 4 e/p/s in CDS mode from Gatan K3

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

1143

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.417, 0.417)

Details:

1143 multi-frame raw micrographs composed of 120 frames each in LZW compressed TIFF format, along with separate gain reference for the 4 e-/pix/s dose rate dataset at 105kx nominal magnification collected in CDS mode of Gatan K3.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

4. apoferritin multi-frame raw micrographs non gain normalized frames in LZW compressed TIFF format collected at 4 e/p/s in CDS mode from Gatan K3

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

1152

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.417, 0.417)

Details:

1152 multi-frame raw micrographs composed of 120 frames each in LZW compressed TIFF format, along with separate gain reference for the 16 e-/pix/s dose rate dataset at 105kx nominal magnification collected in CDS mode of Gatan K3.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

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5. apoferritin multi-frame raw micrographs non gain normalized frames in LZW compressed TIFF format collected at 8 e/p/s in CDS mode from Gatan K3 at lower magnification

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

1080

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.673, 0.673)

Details:

1080 multi-frame raw micrographs composed of 120 frames each in LZW compressed TIFF format, along with separate gain reference for the 8 e-/pix/s dose rate dataset at 64kx nominal magnification collected in CDS mode of Gatan K3.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

6. apoferritin multi-frame raw micrographs non gain normalized frames in LZW compressed TIFF format collected at 8 e/p/s in standard mode from Gatan K3 at lower magnification

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

1107

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.673, 0.673)

Details:

1107 multi-frame raw micrographs composed of 120 frames each in LZW compressed TIFF format, along with separate gain reference for the 8 e-/pix/s dose rate dataset at 64kx nominal magnification collected in standard mode of Gatan K3.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

7. apoferritin multi-frame raw micrographs non gain normalized frames in LZW compressed TIFF format collected at 16 e/p/s in CDS mode from Gatan K3 at lower magnification

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

1152

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.673, 0.673)

Details:

1152 multi-frame raw micrographs composed of 120 frames each in LZW compressed TIFF format, along with separate gain reference for the 16 e-/pix/s dose rate dataset at 64kx nominal magnification collected in CDS mode of Gatan K3.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

8. apoferritin multi-frame raw micrographs non gain normalized frames in LZW compressed TIFF format collected at 16 e/p/s in standard mode from Gatan K3 at lower magnification

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

1143

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.673, 0.673)

Details:

1143 multi-frame raw micrographs composed of 120 frames each in LZW compressed TIFF format, along with separate gain reference for the 16 e-/pix/s dose rate dataset at 64kx nominal magnification collected in standard mode of Gatan K3.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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