EMPIAR-10586 - Cryo electron micrographs of digitonin-solubilized, amphipol-stabilized, sucrose-gradient-purified V. radiata mitochondrial membranes - mixed fraction containing CI*, CIII2 and SC III2+IV [9815 multi-frame micrographs composed of 118 frames each in TIFF format]

EMPIAR-10586

Cryo electron micrographs of digitonin-solubilized, amphipol-stabilized, sucrose-gradient-purified V. radiata mitochondrial membranes - mixed fraction containing CI*, CIII2 and SC III2+IV [9815 multi-frame micrographs composed of 118 frames each in TIFF format]

Publication:

Atomic structure of a mitochondrial complex I intermediate from vascular plants

Maldonado M, Padavannil A, Zhou L, Guo F, Letts JA

eLife 9 (2020)

PMID: 32840211

DOI: 10.7554/elife.56664

Related EMDB entry:

EMD-22448 EMD-22447 EMD-22445 EMD-22450 EMD-22449 EMD-22090 EMD-22093 EMD-22092

Deposited:

2020-11-17

Released:

2020-12-18

Last modified:

2022-05-31

Imageset size:

6.70 TB

Imageset DOI:

10.6019/EMPIAR-10586

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Raw movies of mixed sample used for determination of mung bean respiratory CI*, CIII2, CIV and SC III2+IV

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

9815

Image size:

(5760, 4092)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(0.8332, 0.8332)

Details:

9815 unaveraged, raw movies with 118 frames accumulating 86.4 e/A2 dose over 3 seconds.

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Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

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