EMPIAR-10546
2.05 angstrom resolution structure determination of sulfur oxygenase reductase using 200kV cryo-EM [2558 multi-frame micrographs composed of 50 frames each in MRC format]
Publication:

Crystallographic and cryogenic electron microscopic structures and enzymatic characterization of sulfur oxygenase reductase from Sulfurisphaera tokodaii.

Sato Y, Yabuki T, Adachi N, Moriya T, Arakawa T, Kawasaki M, Yamada C, Senda T, Fushinobu S, Wakagi T

Journal of Structural Biology: X 4 (2020)

PMID: 32775998

Related EMDB entry:
Deposited:
2020-11-03
Released:
2020-11-18
Last modified:
2020-11-18
Imageset size:
1.70 TB
Imageset DOI:
Experimental metadata:
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Original size:   4,191,029,758 kilobytes
Compressed size: 1,829,910,122 kilobytes
Deposited via PDBj
Contains:
  • micrographs - multiframe
1. 2.05 angstrom resolution structure determination of sulfur oxygenase reductase using 200kV cryo-EM
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
2558
Image size:
(4096, 4096)
Pixel type:
SIGNED 16 BIT INTEGER
Pixel spacing:
(0.68, 0.68)
Details:
We started single particle analysis by using the nominal pixel size (0.69A/px). The pixel size was checked by comparing the cryo-EM structure with the atomic-coordinate model of the crystal structure using the 'Fit in Map' tool in UCSF Chimera and was finally calibrated to 0.676 A/pixel
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