EMPIAR-10543
Cryo electron microscopy of RagAB from P. gingivalis solubilised in DDM [3635 multi-frame micrographs composed of 48 frames each in MRC format]
Publication:

Structural and functional insights into oligopeptide acquisition by the RagAB transporter from Porphyromonas gingivalis

Madej M, White JBR, Nowakowska Z, Rawson S, Scavenius C, Enghild JJ, Bereta GP, Pothula K, Kleinekathoefer U, Baslé A, Ranson NA, Potempa J, van den Berg B

Nature microbiology 5 (2020) 1016-1025

PMID: 32393857

Related EMDB entry:
Deposited:
2020-09-28
Released:
2020-11-13
Last modified:
2020-11-13
Imageset size:
1.02 TB
Imageset DOI:
Experimental metadata:
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Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

Movies in this entry were compressed to TIFF by EMPIAR Japan to save space and bandwidth.
The compression is lossless in that all pixel intensities remain exactly the same after decompression.
However, some metadata in the MRC header were lost.
The original files before compression are available from EMPIAR EBI.

Compression was carried out by separating (“un-normalizing”) the gain.
The details of the method are described in
https://relion.readthedocs.io/en/release-3.1/Reference/MovieCompression.html#gain-normalised-movies.

Please apply the gain reference (gain-reference.mrc) during processing.

Original size:   9,704,759,354 kilobytes
Compressed size: 1,095,265,299 kilobytes
Contains:
  • micrographs - multiframe
1. Raw micrograph movies of the RagAB complex solubilised in DDM
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
3635
Image size:
(3838, 3710)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(None, None)
Details:
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Files:
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Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)
Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)
Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)
Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)
Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)