EMPIAR-10517 - Movies of Nsp7-Nsp8-Nsp12 SARS-CoV2 RNA-dependent RNA polymerase in complex with template:primer dsRNA and favipiravir-RTP [63977 multi-frame micrographs composed of 24 frames each in MRCS format]

EMPIAR-10517

Movies of Nsp7-Nsp8-Nsp12 SARS-CoV2 RNA-dependent RNA polymerase in complex with template:primer dsRNA and favipiravir-RTP [63977 multi-frame micrographs composed of 24 frames each in MRCS format]

Publication:

Structure of the SARS-CoV-2 RNA-dependent RNA polymerase in the presence of favipiravir-RTP

Naydenova K, Muir KW, Wu LF, Zhang Z, Coscia F, Peet MJ, Castro-Hartmann P, Qian P, Sader K, Dent K, Kimanius D, Sutherland JD, Löwe J, Barford D, Russo CJ

Proceedings of the National Academy of Sciences of the United States of America 118 (2021)

PMID: 33526596

DOI: 10.1073/pnas.2021946118

Related EMDB entry:

EMD-11692

Deposited:

2020-09-02

Released:

2020-11-27

Last modified:

2022-01-31

Imageset size:

23.95 TB

Imageset DOI:

10.6019/EMPIAR-10517

Experimental metadata:

Download xml json

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

MRC movies (*.mrcs) in this entry were compressed by bzip2 (pbzip2)
by EMPIAR Japan to save space and bandwidth.

Some programs (e.g. RELION >= 4.0.1 and CryoSPARC) can process compressed movies without explicit
decompression. For other programs, movies have to be decompressed beforehand. In this case,
you might want to convert decompressed MRC movies into TIFF to save your local storage.

Original size:   50,312,870,397 kilobytes
Compressed size: 25,714,347,957 kilobytes

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of SARS-CoV-2 RNA-dependent RNA polymerase in compex with template:primer dsRNA and favipiravir-RTP

Category:

micrographs - multiframe

Image format:

MRCS

No. of images or tilt series:

63977

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(0.65, 0.65)

Details:

The micrographs are split in directories for each grid used for data collection: three HexAuFoil grids with 260 nm holes and one graphene-coated UltrAuFoil R0.6/1 grid.

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Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

Structural basis for HIV-1 antagonism of host APOBEC3G via Cullin E3 ligase.

Ito F, Alvarez-Cabrera AL, Liu S, Yang H, Shiriaeva A, Zhou ZH, Chen XS. (2023)

The 2023 Nucleic Acids Research Database Issue and the online molecular biology database collection.

Rigden DJ, Fernández XM. (2023)

EMPIAR: the Electron Microscopy Public Image Archive.

Iudin A, Korir PK, Somasundharam S, Weyand S, Cattavitello C, Fonseca N, Salih O, Kleywegt GJ, Patwardhan A. (2023)

CLEMSite, a software for automated phenotypic screens using light microscopy and FIB-SEM.

Serra Lleti JM, Steyer AM, Schieber NL, Neumann B, Tischer C, Hilsenstein V, Holtstrom M, Unrau D, Kirmse R, Lucocq JM, Pepperkok R, Schwab Y. (2023)

Structure of a RecT/Redβ family recombinase in complex with a duplex intermediate of DNA annealing.

Caldwell BJ, Norris AS, Karbowski CF, Wiegand AM, Wysocki VH, Bell CE. (2022)

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