EMPIAR-10513 - mouse cGAS with reconstituted nucleosome [5353 multi-frame micrographs composed of 40 frames each in MRC format]

EMPIAR-10513

mouse cGAS with reconstituted nucleosome [5353 multi-frame micrographs composed of 40 frames each in MRC format]

Publication:

The Molecular Basis of Tight Nuclear Tethering and Inactivation of cGAS

Zhao B, Xu P, Rowlett CM, Jing T, Shinde O, Lei Y, West AP, Liu WR, Li P

Nature (2020)

PMID: 32911481

DOI: 10.1038/s41586-020-2749-z

Related EMDB entry:

EMD-22046

Deposited:

2020-09-18

Released:

2020-10-09

Last modified:

2020-10-09

Imageset size:

824.25 GB

Imageset DOI:

10.6019/EMPIAR-10513

Experimental metadata:

Download xml json

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

MRC movies (FoilHole*.mrc) in this entry were compressed by bzip2 (pbzip2)
by EMPIAR Japan to save space and bandwidth.

Some programs (e.g. RELION >= 4.0.1 and CryoSPARC) can process compressed movies without explicit
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Original size:   2,976,843,949 kilobytes
Compressed size:   804,875,547 kilobytes

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multiframe micrographs of mouse cGAS / human reconstituted nucleosomes

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

5353

Image size:

(3838, 3710)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.07, 1.07)

Details:

---

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Citations

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CryoEMNet driven symmetry-aware molecular reconstruction through deep learning enhanced electron microscopy.

Arora S, Pan SH, Kumar S, Singh SK, Chauhan U, Alhalabi W, Arya V, Alsulami BS, Hsu CH, Chui KT, Gupta BB. (2025)

PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

Costa-Gomes B, Greer J, Juraschko N, Parkhurst J, Mirecka J, Famili M, Rangel-Smith C, Strickson O, Lowe A, Basham M, Burnley T. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

Laser flash melting cryo-EM samples to overcome preferred orientation.

Straub MS, Harder OF, Mowry NJ, Barrass SV, Hruby J, Drabbels M, Lorenz UJ. (2025)

In situ structure determination of conformationally flexible targets using nextPYP.

Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)

EMICSS: added-value annotations for EMDB entries.

Duraisamy AK, Fonseca N, Kleywegt GJ, Patwardhan A, Morris KL. (2025)

Global conformations of <i>Pichia pastoris</i> complex I are distinguished by the binding of a unique interdomain bridging subunit.

Lee CS, Grba DN, Wright JJ, Ivanov BS, Hirst J. (2025)

Overcoming Preferred Orientation in Cryo-EM With Ultrasonic Excitation During Vitrification

Williams HM, Curtis WA, Haubner M, Hruby J, Drabbels M, Lorenz UJ. (2025)

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