EMPIAR-10507 - Structure of human Frizzled5 by fiducial-assisted cryo-EM [10922 multi-frame micrographs composed of 50 frames each in TIFF format]

EMPIAR-10507

Structure of human Frizzled5 by fiducial-assisted cryo-EM [10922 multi-frame micrographs composed of 50 frames each in TIFF format]

Publication:

Structure of human Frizzled5 by fiducial-assisted cryo-EM supports a heterodimeric mechanism of canonical Wnt signaling

Tsutsumi N, Mukherjee S, Waghray D, Janda CY, Jude KM, Miao Y, Burg JS, Aduri NG, Kossiakoff AA, Gati C, Garcia KC

eLife (2020)

PMID: 32762848

DOI: 10.7554/eLife.58464

Related EMDB entry:

EMD-21927

Deposited:

2020-08-27

Released:

2020-10-16

Last modified:

2020-10-16

Imageset size:

6.05 TB

Imageset DOI:

10.6019/EMPIAR-10507

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned dark-subtracted TIFF movies with a gain reference for the 3D reconstruction of EMD-21927.

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

10922

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.539, 0.539)

Details:

Set defocus range: -0.8 um to -2.0 um
Exposure rate at specimen: 9.5 e/pix/s
Total exposure: 50 e over 6.125 s (0.1225 s/frame)
Exposure rate at camera: 7.5~8.6 e/pix/s (6.45~ e/A2/s, 0.8~ e/A2/frame)

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Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

CryoTRANS: predicting high-resolution maps of rare conformations from self-supervised trajectories in cryo-EM.

Fan X, Zhang Q, Zhang H, Zhu J, Ju L, Shi Z, Hu M, Bao C. (2024)

Self-assembled superstructure alleviates air-water interface effect in cryo-EM.

Zheng L, Xu J, Wang W, Gao X, Zhao C, Guo W, Sun L, Cheng H, Meng F, Chen B, Sun W, Jia X, Zhou X, Wu K, Liu Z, Ding F, Liu N, Wang HW, Peng H. (2024)

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