EMPIAR-10502 - CryoEM structure of EPYC1(49-72) peptide-bound Rubisco [2500 multi-frame micrographs composed of 50 frames each in TIFF format]

EMPIAR-10502

CryoEM structure of EPYC1(49-72) peptide-bound Rubisco [2500 multi-frame micrographs composed of 50 frames each in TIFF format]

Publication:

The structural basis of Rubisco phase separation in the pyrenoid

He S, Chou HT, Matthies D, Wunder T, Meyer MT, Atkinson N, Martinez-Sanchez A, Jeffrey PD, Port SA, Patena W, He G, Chen VK, Hughson FM, McCormick AJ, Mueller-Cajar O, Engel BD, Yu Z, Jonikas MC

Nature plants 6 (2020) 1480-1490

PMID: 33230314

DOI: 10.1038/s41477-020-00811-y

Related EMDB entry:

EMD-22308

Deposited:

2020-08-14

Released:

2020-11-23

Last modified:

2022-02-08

Imageset size:

1.31 TB

Imageset DOI:

10.6019/EMPIAR-10502

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multuframe micrographs of EPYC1(49-72) peptide-bound Rubisco

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

2500

Image size:

(7676, 7420)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.655, 0.655)

Details:

The movies are recorded on K2 camera in super resolution mode. The electron dose is 10 electron/pixel/sec and the total exposure time is 10 sec. Each movie contains 50 frames.

Files:

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Citations

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Kim K, Li H, Clarke OB. (2025)

CLEM-Reg: an automated point cloud-based registration algorithm for volume correlative light and electron microscopy.

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CryoEMNet driven symmetry-aware molecular reconstruction through deep learning enhanced electron microscopy.

Arora S, Pan SH, Kumar S, Singh SK, Chauhan U, Alhalabi W, Arya V, Alsulami BS, Hsu CH, Chui KT, Gupta BB. (2025)

PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

Costa-Gomes B, Greer J, Juraschko N, Parkhurst J, Mirecka J, Famili M, Rangel-Smith C, Strickson O, Lowe A, Basham M, Burnley T. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

Laser flash melting cryo-EM samples to overcome preferred orientation.

Straub MS, Harder OF, Mowry NJ, Barrass SV, Hruby J, Drabbels M, Lorenz UJ. (2025)

In situ structure determination of conformationally flexible targets using nextPYP.

Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)

EMICSS: added-value annotations for EMDB entries.

Duraisamy AK, Fonseca N, Kleywegt GJ, Patwardhan A, Morris KL. (2025)

Global conformations of <i>Pichia pastoris</i> complex I are distinguished by the binding of a unique interdomain bridging subunit.

Lee CS, Grba DN, Wright JJ, Ivanov BS, Hirst J. (2025)

Overcoming Preferred Orientation in Cryo-EM With Ultrasonic Excitation During Vitrification

Williams HM, Curtis WA, Haubner M, Hruby J, Drabbels M, Lorenz UJ. (2025)

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