EMPIAR-10500
High resolution structure of GABAAR beta3 homopentamer [multiple data sets in EER and TIFF formats]
Publication:

Single-particle cryo-EM at atomic resolution

Nakane T, Kotecha A, Sente A, McMullan G, Masiulis S, Brown PMGE, Grigoras IT, Malinauskaite L, Malinauskas T, Miehling J, Uchański T, Yu L, Karia D, Pechnikova EV, de Jong E, Keizer J, Bischoff M, McCormack J, Tiemeijer P, Hardwick SW, Chirgadze DY, Murshudov G, Aricescu AR, Scheres SHW

Nature (2020)

PMID: 33087931

Related EMDB entry:
Deposited:
2020-05-24
Released:
2020-11-13
Last modified:
2020-11-13
Imageset size:
13.05 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
1. CFEG, Falcon4, with 5 eV filter
Category:
micrographs - multiframe
Image format:
EER
No. of images or tilt series:
5573
Image size:
(4096, 4096)
Pixel type:
BIT
Pixel spacing:
(0.727, 0.727)
Details:
Total dose: 40 e/A2 on sample

Please use gainImage_8k_defMasked.mrc as the gain reference without any transformation. Note that the EER gain reference has different convention from those for K2/K3 cameras. That is, the raw values must be divided, not multiplied, by the gain. RELION automatically handles this.
Files:
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2. CFEG, Falcon4, with 5 eV filter, without objective aperture
Category:
micrographs - multiframe
Image format:
EER
No. of images or tilt series:
3160
Image size:
(4096, 4096)
Pixel type:
BIT
Pixel spacing:
(0.727, 0.727)
Details:
Same cautions as "CFEG, Falcon4, with 5 eV filter" apply.

Please use gainImage_8k_defMasked.mrc as the gain reference without any transformation. Note that the EER gain reference has different convention from those for K2/K3 cameras. That is, the raw values must be divided, not multiplied, by the gain. RELION automatically handles this.
Files:
Loading...
3. XFEG, Falcon3, without filter
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
1501
Image size:
(4096, 4096)
Pixel type:
SIGNED 16 BIT INTEGER
Pixel spacing:
(0.827, 0.827)
Details:
The pixel size in the image header (0.8583 A/px) is wrong. The processing was started with
a nominal pixel size of 0.83 A/px, which was later calibrated to 0.827 A/px.

Dose rate: 0.93 e/A2/frame
Files:
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4. XFEG, Falcon4, with filter but retracted slit
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
1244
Image size:
(4096, 4096)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(0.735, 0.735)
Details:
The pixel size in the header (1.00 A/px) is wrong. Please ignore it.

Dose rate: 0.44 e/A2/frame
Files:
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5. XFEG, Falcon4, with 3 eV filter
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
1952
Image size:
(4096, 4096)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(0.735, 0.735)
Details:
Same cautions as "XFEG, Falcon4, with filter but retracted slit" apply.
Files:
Loading...
6. XFEG, Falcon4, with 5 eV filter
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
1556
Image size:
(4096, 4096)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(0.735, 0.735)
Details:
Same cautions as "XFEG, Falcon4, with filter but retracted slit" apply.
Files:
Loading...
7. XFEG, Falcon4, with 3 eV filter
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
1268
Image size:
(4096, 4096)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(0.735, 0.735)
Details:
Same cautions as "XFEG, Falcon4, with filter but retracted slit" apply.
Files:
Loading...
8. XFEG, K3, without filter
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
1508
Image size:
(11520, 8184)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.32741, 0.32741)
Details:
The pixel size in the image header is wrong. The processing was started with
a nominal (super-resolution) pixel size of 0.325 A/px, which was later calibrated to 0.32741 A/px. Please use the provided gain reference (gain.mrc) without any transformation.

Dose rate: 0.96 e/A2/frame
Files:
Loading...
Files:
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