EMPIAR-10492
Cryo-EM structure of SARS-CoV-2 Spike Proteins on intact virions [7982 multi-frame micrographs composed of 48 frames each in TIFF format]
Publication:

Structures and distributions of SARS-CoV-2 spike proteins on intact virions.

Ke Z, Oton J, Qu K, Cortese M, Zila V, McKeane L, Nakane T, Zivanov J, Neufeldt CJ, Cerikan B, Lu JM, Peukes J, Xiong X, Kräusslich HG, Scheres SHW, Bartenschlager R, Briggs JAG

Nature (2020)

PMID: 32805734

Related EMDB entry:
Deposited:
2020-08-05
Released:
2020-08-19
Last modified:
2020-08-19
Imageset size:
2.07 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
1. Unaligned movies
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
7982
Image size:
(5760, 4092)
Pixel type:
SIGNED BYTE
Pixel spacing:
(1.061, 1.061)
Details:
50 e/A2 in 48 frames.

We started data processing at the nominal pixel size of 1.098 A/px. Thus, the pixel size and CTF parameters in image headers and STAR files assume the nominal 1.098 A/px. We updated to the calibrated pixel size of 1.061 A/px in PostProcess.

Please use CountRef_SARS2_HGKA54_ZK144_G2_K3_0000.mrc as the gain reference with "Rotation of 180 degrees (2)" and "Flip left to right (2)".

The "Polished" folder contains the polished particles. We also deposited three STAR files containing final refined parameters.
- job095_run_data.star: the "2 closed + 1 weak RBDs" state
- job096_run_data.star: the "3 closed RBDs" state
- job055_run_data.star: both
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