EMPIAR-10492 - Cryo-EM structure of SARS-CoV-2 Spike Proteins on intact virions [7982 multi-frame micrographs composed of 48 frames each in TIFF format]

EMPIAR-10492

Cryo-EM structure of SARS-CoV-2 Spike Proteins on intact virions [7982 multi-frame micrographs composed of 48 frames each in TIFF format]

Publication:

Structures and distributions of SARS-CoV-2 spike proteins on intact virions.

Ke Z, Oton J, Qu K, Cortese M, Zila V, McKeane L, Nakane T, Zivanov J, Neufeldt CJ, Cerikan B, Lu JM, Peukes J, Xiong X, Kräusslich HG, Scheres SHW, Bartenschlager R, Briggs JAG

Nature (2020)

PMID: 32805734

DOI: 10.1038/s41586-020-2665-2

Related EMDB entry:

EMD-11497 EMD-11498

Deposited:

2020-08-05

Released:

2020-08-19

Last modified:

2020-08-19

Imageset size:

2.07 TB

Imageset DOI:

10.6019/EMPIAR-10492

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned movies

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

7982

Image size:

(5760, 4092)

Pixel type:

SIGNED BYTE

Pixel spacing:

(1.061, 1.061)

Details:

50 e/A2 in 48 frames.

We started data processing at the nominal pixel size of 1.098 A/px. Thus, the pixel size and CTF parameters in image headers and STAR files assume the nominal 1.098 A/px. We updated to the calibrated pixel size of 1.061 A/px in PostProcess.

Please use CountRef_SARS2_HGKA54_ZK144_G2_K3_0000.mrc as the gain reference with "Rotation of 180 degrees (2)" and "Flip left to right (2)".

The "Polished" folder contains the polished particles. We also deposited three STAR files containing final refined parameters.
- job095_run_data.star: the "2 closed + 1 weak RBDs" state
- job096_run_data.star: the "3 closed RBDs" state
- job055_run_data.star: both

Files:

Archives and downloads the selected files into an uncompressed zip file.

Depending on the number and size of files to be downloaded, this can take an enormous amount of time. Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

Quick Links

Talks and Tutorials

EMPIAR Quick tour

Statistics

Publications

Re-use case study

EMPIAR in the news

Claim entries to your ORCiD

COVID-19 Data Portal

EMDB

PDBe

BioImage Archive

EMNavigator@PDBj

PDBj

RCSB@PDBj

EMDataResoruce@RCSB,EMDB,NCMI

Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

See all citations

Protein Data Bank Japan News

[2024-11-20]

224 new PDB entries have been released on 2024-11-20.

[2024-11-01]

A new portal integrating genome variants and protein structures has released

[2024-10-30]

[wwPDB] Deprecation of FTP File Download Protocol on November 1, 2024

[2024-10-25]

PDBj luncheon seminar at APBJC 2024

[2024-10-17]

[wwPDB] The Nobel Prize in Chemistry 2024