EMPIAR-10472 - CryoEM structure of human CMG bound to AND-1 (CMGA) [1844 multi-frame micrographs composed of 23 frames each in MRC format]

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EMPIAR-10472

CryoEM structure of human CMG bound to AND-1 (CMGA) [1844 multi-frame micrographs composed of 23 frames each in MRC format]

Publication:

CryoEM structures of human CMG-ATPγS-DNA and CMG-AND-1 complexes

Rzechorzek NJ, Hardwick SW, Jatikusumo VA, Chirgadze DY, Pellegrini L

Nucleic acids research 48 (2020) 6980-6995

PMID: 32453425

DOI: 10.1093/nar/gkaa429

Related EMDB entry:

EMD-10621

Deposited:

2020-05-02

Released:

2020-08-18

Last modified:

2020-08-18

Imageset size:

1.42 TB

Imageset DOI:

10.6019/EMPIAR-10472

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multiframe micrographs of human CMG bound to AND-1 (CMGA)

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

1844

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(1.06, 1.06)

Details:

Corrected, unaligned integrating mode frame stacks (23 frames) of the human CMG helicase bound to AND-1 (CMGA), collected on a Titan Krios with an FEI Falcon 3 direct electron detector.

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Citations

High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles

Kim K, Li H, Clarke OB. (2025)

CLEM-Reg: an automated point cloud-based registration algorithm for volume correlative light and electron microscopy.

Krentzel D, Elphick M, Domart MC, Peddie CJ, Laine RF, Shand C, Henriques R, Collinson LM, Jones ML. (2025)

CryoEMNet driven symmetry-aware molecular reconstruction through deep learning enhanced electron microscopy.

Arora S, Pan SH, Kumar S, Singh SK, Chauhan U, Alhalabi W, Arya V, Alsulami BS, Hsu CH, Chui KT, Gupta BB. (2025)

PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

Costa-Gomes B, Greer J, Juraschko N, Parkhurst J, Mirecka J, Famili M, Rangel-Smith C, Strickson O, Lowe A, Basham M, Burnley T. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

Laser flash melting cryo-EM samples to overcome preferred orientation.

Straub MS, Harder OF, Mowry NJ, Barrass SV, Hruby J, Drabbels M, Lorenz UJ. (2025)

In situ structure determination of conformationally flexible targets using nextPYP.

Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)

EMICSS: added-value annotations for EMDB entries.

Duraisamy AK, Fonseca N, Kleywegt GJ, Patwardhan A, Morris KL. (2025)

Global conformations of <i>Pichia pastoris</i> complex I are distinguished by the binding of a unique interdomain bridging subunit.

Lee CS, Grba DN, Wright JJ, Ivanov BS, Hirst J. (2025)

Overcoming Preferred Orientation in Cryo-EM With Ultrasonic Excitation During Vitrification

Williams HM, Curtis WA, Haubner M, Hruby J, Drabbels M, Lorenz UJ. (2025)

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