EMPIAR-10465
Single-particle cryo-EM dataset of the complex between a nucleosome and the regulatory linker of ALC1 [multiple data sets in TIFF and MRCS formats]
Publication:

Mechanistic Insights into Regulation of the ALC1 Remodeler by the Nucleosome Acidic Patch

Lehman LC, Bacic L, Hewitt G, Brackmann K, Sabantsev A, Gaullier G, Pytharopoulou S, Degliesposti G, Okkenhaug H, Tan S, Costa A, Skehel JM, Boulton SJ, Deindl S

Cell reports 33 (2020)

PMID: 33357431

Related EMDB entry:
Deposited:
2020-06-26
Released:
2021-01-29
Last modified:
2021-01-29
Imageset size:
6.84 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
  • picked particles - multiframe - processed
1. Unaligned movies of the nucleosome - ALC1-linker complex - session_1_disk_1
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
2852
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.654, 0.654)
Details:
60 gain-corrected frames per movie, collected on Titan Krios with K3 in counting mode.
Files:
Loading...
2. Unaligned movies of the nucleosome - ALC1-linker complex - session_1_disk_2
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
5984
Image size:
(5760, 4096)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.654, 0.654)
Details:
60 gain-corrected frames per movie, collected on Titan Krios with K3 in counting mode.
Files:
Loading...
3. Unaligned movies of the nucleosome - ALC1-linker complex - session_1_disk_3
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
2287
Image size:
(5760, 4096)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.654, 0.654)
Details:
60 gain-corrected frames per movie, collected on Titan Krios with K3 in counting mode.
Files:
Loading...
4. Unaligned movies of the nucleosome - ALC1-linker complex - session_2_grid_1
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
6155
Image size:
(5760, 4096)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.654, 0.654)
Details:
60 gain-corrected frames per movie, collected on Titan Krios with K3 in counting mode.
Files:
Loading...
5. Unaligned movies of the nucleosome - ALC1-linker complex - session_2_grid_2
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
2619
Image size:
(5760, 4096)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.654, 0.654)
Details:
60 gain-corrected frames per movie, collected on Titan Krios with K3 in counting mode.
Files:
Loading...
6. Particles of the high-resolution nucleosome class
Category:
picked particles - multiframe - processed
Image format:
MRCS
No. of images or tilt series:
636505
Image size:
(300, 300)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.654, 0.654)
Details:
Particles from final Refine3D job in RELION-3.1-5857e1 for the high-resolution nucleosome class.
Files:
Loading...
7. Particles of the hexasome class
Category:
picked particles - multiframe - processed
Image format:
MRCS
No. of images or tilt series:
414605
Image size:
(300, 300)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.654, 0.654)
Details:
Particles from final Refine3D job in RELION-3.1-5857e1 for the hexasome class.
Files:
Loading...
Files:
Loading...

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