EMPIAR-10400 - Cryo-EM structure of mouse TRPV3 in a nanodisc [1818 multi-frame micrographs composed of 60 frames each in TIFF format]

EMPIAR-10400

Cryo-EM structure of mouse TRPV3 in a nanodisc [1818 multi-frame micrographs composed of 60 frames each in TIFF format]

Publication:

The structure of lipid nanodisc-reconstituted TRPV3 reveals the gating mechanism

Shimada H, Kusakizako T, Dung Nguyen TH, Nishizawa T, Hino T, Tominaga M, Nureki O

Nature structural & molecular biology 27 (2020) 645-652

PMID: 32572254

DOI: 10.1038/s41594-020-0439-z

Related EMDB entry:

EMD-0882

Deposited:

2020-04-20

Released:

2020-11-18

Last modified:

2020-11-18

Imageset size:

2.28 TB

Imageset DOI:

10.6019/EMPIAR-10400

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned movies for mouse TRPV3 in a nanodisc

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

1818

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(0.8346, 0.8346)

Details:

Unaligned, gain corrected movies from Falcon 3 in the counting mode. The MRC files from EPU were converted to the TIFF format by mrc2tif in IMOD. During this process, the pixel size in the image header became 1.0. Note that we started processing with the nominal pixel size of 0.861 Å and finally changed the value in PostProcess so that the pixels had the calibrated size of 0.8346 Å.

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Citations

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PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

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