EMPIAR-10391 - Single-Particle Cryo-EM of Arabinofuranosyltransferase AftD from Mycobacteria, Mutant R1389S [multiple data sets in TIFF, MRC and MRCS formats]

EMPIAR-10391

Single-Particle Cryo-EM of Arabinofuranosyltransferase AftD from Mycobacteria, Mutant R1389S [multiple data sets in TIFF, MRC and MRCS formats]

Publication:

Cryo-EM Structures and Regulation of Arabinofuranosyltransferase AftD from Mycobacteria

Tan YZ, Zhang L, Rodrigues J, Zheng RB, Giacometti SI, Rosário AL, Kloss B, Dandey VP, Wei H, Brunton R, Raczkowski AM, Athayde D, Catalão MJ, Pimentel M, Clarke OB, Lowary TL, Archer M, Niederweis M, Potter CS, Carragher B, Mancia F

Molecular cell (2020)

PMID: 32386575

DOI: 10.1016/j.molcel.2020.04.014

Related EMDB entry:

EMD-21601 EMD-21600

Deposited:

2020-04-12

Released:

2020-05-29

Last modified:

2020-05-29

Imageset size:

2.28 TB

Imageset DOI:

10.6019/EMPIAR-10391

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
micrographs - single frame
picked particles - single frame - processed

Image Sets

1. Unaligned and compressed multi-frame movies

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

4889

Image size:

(3838, 3710)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(1.0605, 1.0605)

Details:

Data collection was performed using a dose of ~96.78 e-/Å2 across 90 frames (150 msec per frame) at a dose rate of ~8.34 e-/pix/sec, using a set defocus range of -0.8 μm to -1.6 μm. Raw frames were compressed using pbzip2. Gain reference image is 01_Raw_Frames/18nov03b/rawdata/references/18nov02f_03102315_01_3838x3710_norm_0_mod.mrc.

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2. Aligned and dose-weighted micrographs

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

4888

Image size:

(3838, 3710)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.0605, 1.0605)

Details:

Movie frames were aligned using MotionCor2 with 5 by 5 patches and B-factor of 100 through the Appion software package.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

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3. Final Particle Stacks with Refined Euler Angles and Shifts (Overall and Focused Refined on CBM3)

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

Image size:

(256, 256)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.0605, 1.0605)

Details:

Final particle stacks, with Relion .star file containing the refined Euler angles and shifts. AftD-R1389S_All-Particles comprise of all particles while AftD-R1389S_Class1 comprise of particles going into Class 1 and AftD-R1389S_Class2 comprise of particles going into Class 2.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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