EMPIAR-10383 - CryoEM structure of human LRRC8D [3399 multi-frame micrographs composed of 40 frames each in TIFF format]

EMPIAR-10383

CryoEM structure of human LRRC8D [3399 multi-frame micrographs composed of 40 frames each in TIFF format]

Publication:

Cryo-EM structure of the volume-regulated anion channel LRRC8D isoform identifies features important for substrate permeation

Nakamura R, Numata T, Kasuya G, Yokoyama T, Nishizawa T, Kusakizako T, Kato T, Hagino T, Dohmae N, Inoue M, Watanabe K, Ichijo H, Kikkawa M, Shirouzu M, Jentsch TJ, Ishitani R, Okada Y, Nureki O

Communications biology 3 (2020)

PMID: 32415200

DOI: 10.1038/s42003-020-0951-z

Related EMDB entry:

EMD-30029

Deposited:

2020-03-05

Released:

2021-11-09

Last modified:

2023-05-02

Imageset size:

1.88 TB

Imageset DOI:

10.6019/EMPIAR-10383

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned movies of human LRRC8D isoform

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

3399

Image size:

(7420, 7676)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.49, 1.49)

Details:

Movies were collected on Talos Arctica at 0.745 A / super-resolution pixel (that is, 1.49 A / physical pixel). Gain references must be rotated and flipped. In MotionCor2, this is "-RotGain 1 -FlipGain 2". Seven datasets were combined to yield the structure.
Dose rate (shown on SerialEM) of Datasets 0 and 1 are 1.245 e/A2/frame

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Citations

High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles

Kim K, Li H, Clarke OB. (2025)

CLEM-Reg: an automated point cloud-based registration algorithm for volume correlative light and electron microscopy.

Krentzel D, Elphick M, Domart MC, Peddie CJ, Laine RF, Shand C, Henriques R, Collinson LM, Jones ML. (2025)

CryoEMNet driven symmetry-aware molecular reconstruction through deep learning enhanced electron microscopy.

Arora S, Pan SH, Kumar S, Singh SK, Chauhan U, Alhalabi W, Arya V, Alsulami BS, Hsu CH, Chui KT, Gupta BB. (2025)

PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

Costa-Gomes B, Greer J, Juraschko N, Parkhurst J, Mirecka J, Famili M, Rangel-Smith C, Strickson O, Lowe A, Basham M, Burnley T. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

Laser flash melting cryo-EM samples to overcome preferred orientation.

Straub MS, Harder OF, Mowry NJ, Barrass SV, Hruby J, Drabbels M, Lorenz UJ. (2025)

In situ structure determination of conformationally flexible targets using nextPYP.

Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)

EMICSS: added-value annotations for EMDB entries.

Duraisamy AK, Fonseca N, Kleywegt GJ, Patwardhan A, Morris KL. (2025)

Global conformations of <i>Pichia pastoris</i> complex I are distinguished by the binding of a unique interdomain bridging subunit.

Lee CS, Grba DN, Wright JJ, Ivanov BS, Hirst J. (2025)

Overcoming Preferred Orientation in Cryo-EM With Ultrasonic Excitation During Vitrification

Williams HM, Curtis WA, Haubner M, Hruby J, Drabbels M, Lorenz UJ. (2025)

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