EMPIAR-10351 - Cryo-EM structure of the human PAC1 receptor coupled to an engineered heterotrimeric G protein [2895 multi-frame micrographs composed of 64 frames each in TIFF format]

EMPIAR-10351

Cryo-EM structure of the human PAC1 receptor coupled to an engineered heterotrimeric G protein [2895 multi-frame micrographs composed of 64 frames each in TIFF format]

Publication:

Cryo-EM structure of the human PAC1 receptor coupled to an engineered heterotrimeric G protein

Kobayashi K, Shihoya W, Nishizawa T, Kadji FMN, Aoki J, Inoue A, Nureki O

Nature structural & molecular biology 27 (2020) 274-280

PMID: 32157248

DOI: 10.1038/s41594-020-0386-8

Related EMDB entry:

EMD-0940

Deposited:

2020-01-31

Released:

2020-04-03

Last modified:

2020-02-04

Imageset size:

3.52 TB

Imageset DOI:

10.6019/EMPIAR-10351

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned movies for the human PAC1 receptor coupled to Gs (dimer)

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

2895

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(0.8346, 0.8346)

Details:

Unaligned, gain corrected movies from Falcon 3 in the counting mode. The MRC files from EPU were converted to the TIFF format by mrc2tif in IMOD. During this process, the pixel size in the image header became 1.0. Note that we started processing with the nominal pixel size of 0.861 Å and finally changed the value in PostProcess so that the pixels had the calibrated size of 0.8346 Å.

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