EMPIAR-10346 - Cryo-EM of GLP-1 receptor bound to TT-OAD2 non-peptidic agonist [multiple data sets in TIFF format]

EMPIAR-10346

Cryo-EM of GLP-1 receptor bound to TT-OAD2 non-peptidic agonist [multiple data sets in TIFF format]

Publication:

Activation of the GLP-1 receptor by a non-peptidic agonist

Zhao P, Liang YL, Belousoff MJ, Deganutti G, Fletcher MM, Willard FS, Bell MG, Christe ME, Sloop KW, Inoue A, Truong TT, Clydesdale L, Furness SGB, Christopoulos A, Wang MW, Miller LJ, Reynolds CA, Danev R, Sexton PM, Wootten D

Nature 577 (2020) 432-436

PMID: 31915381

DOI: 10.1038/s41586-019-1902-z

Related EMDB entry:

EMD-20179

Deposited:

2020-01-09

Released:

2020-03-10

Last modified:

2020-03-10

Imageset size:

6.25 TB

Imageset DOI:

10.6019/EMPIAR-10346

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame movies of GLP-1R-TT-OAD2 complex

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

4259

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.413, 0.413)

Details:

- Approximately two-thirds of the data were collected with the Volta phase plate (VPP). "VPP" in the filename indicates that the movie was collected with the phase plate.
- Most movies were collected with a 9-hole beam-image shift acquisition pattern. The filename of such movies contains a four digit number in the range 0000 to 0008 corresponding to the image-shift acquisition position.
- The image-shift induced beam-tilt was not compensated optically during acquisition. For best results, perform per-image-shift position beam-tilt measurement and correction with CTF refinement.
- The movies were recorded with Gatan K3 camera in super-resolution mode and saved as gain-normalized LZW compressed TIFF files by SerialEM.
- Accelerating voltage: 300 kV
- Spherical aberration: 2.7 mm
- SuperRes pixel size: 0.413 A/superpixel
- Physical pixel size: 0.826 A/physpix
- Exposure rate: 10.9 e/physpix/s
- Exposure rate: 16.0 e/A^2/s
- Exposure time: 4.015 s
- Total exposure: 64.2 e/A^2
- Number of frames: 67
- Defocus range: 0.3 - 1.4 um

Files:

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2. Unaligned multi-frame movies of GLP-1R-TT-OAD2 complex

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

3159

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.826, 0.826)

Details:

- The movies were recorded with Gatan K3 camera in counting (non-superres) mode and saved as gain-normalized LZW compressed TIFF files by SerialEM.
- All movies were collected with a 9-hole beam-image shift acquisition pattern. The filename contains a four digit number in the range 0000 to 0008 corresponding to the image-shift acquisition position.
- The image-shift induced beam-tilt was not compensated optically during acquisition. For best results, perform per-image-shift position beam-tilt measurement and correction with CTF refinement.
- Accelerating voltage: 300 kV
- Spherical aberration: 2.7 mm
- Pixel size: 0.826 A/pix
- Exposure rate: 12.2 e/physpix/s
- Exposure rate: 17.9 e/A^2/s
- Exposure time: 3.715 s
- Total exposure: 66.5 e/A^2
- Number of frames: 62
- Defocus range: 0.6 - 1.5 um

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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