EMPIAR-10336 - Structure of two nucleosomes bridged by human PARP2 [multiple data sets in MRCS format]

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EMPIAR-10336

Structure of two nucleosomes bridged by human PARP2 [multiple data sets in MRCS format]

Publication:

Bridging of nucleosome-proximal DNA double-strand breaks by PARP2 enhances its interaction with HPF1

Gaullier G, Roberts G, Muthurajan UM, Bowerman S, Rudolph J, Mahadevan J, Jha A, Rae PS, Luger K

PloS one 15 (2020)

PMID: 33141820

DOI: 10.1371/journal.pone.0240932

Related EMDB entry:

EMD-20864

Deposited:

2019-11-11

Released:

2020-10-09

Last modified:

2021-06-30

Imageset size:

727.25 GB

Imageset DOI:

10.6019/EMPIAR-10336

Experimental metadata:

Download xml json

Contains:

picked particles - single frame - unprocessed
micrographs - multiframe

Image Sets

1. Single-particle images of the PARP2-nucleosome complex

Category:

picked particles - single frame - unprocessed

Image format:

MRCS

No. of images or tilt series:

27889

Image size:

(256, 256)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.271, 1.271)

Details:

Particles used for 3D refinement and post-processing in RELION.

Files:

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2. Unaligned multi-frame micrographs of the PARP2-nucleosome complex

Category:

micrographs - multiframe

Image format:

MRCS

No. of images or tilt series:

632

Image size:

(3838, 3710)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(1.271, 1.271)

Details:

Raw movies.

Files:

This entry cannot be downloaded from PDBj.

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Citations

High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles

Kim K, Li H, Clarke OB. (2025)

CLEM-Reg: an automated point cloud-based registration algorithm for volume correlative light and electron microscopy.

Krentzel D, Elphick M, Domart MC, Peddie CJ, Laine RF, Shand C, Henriques R, Collinson LM, Jones ML. (2025)

CryoEMNet driven symmetry-aware molecular reconstruction through deep learning enhanced electron microscopy.

Arora S, Pan SH, Kumar S, Singh SK, Chauhan U, Alhalabi W, Arya V, Alsulami BS, Hsu CH, Chui KT, Gupta BB. (2025)

PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

Costa-Gomes B, Greer J, Juraschko N, Parkhurst J, Mirecka J, Famili M, Rangel-Smith C, Strickson O, Lowe A, Basham M, Burnley T. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

Laser flash melting cryo-EM samples to overcome preferred orientation.

Straub MS, Harder OF, Mowry NJ, Barrass SV, Hruby J, Drabbels M, Lorenz UJ. (2025)

In situ structure determination of conformationally flexible targets using nextPYP.

Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)

EMICSS: added-value annotations for EMDB entries.

Duraisamy AK, Fonseca N, Kleywegt GJ, Patwardhan A, Morris KL. (2025)

Global conformations of <i>Pichia pastoris</i> complex I are distinguished by the binding of a unique interdomain bridging subunit.

Lee CS, Grba DN, Wright JJ, Ivanov BS, Hirst J. (2025)

Overcoming Preferred Orientation in Cryo-EM With Ultrasonic Excitation During Vitrification

Williams HM, Curtis WA, Haubner M, Hruby J, Drabbels M, Lorenz UJ. (2025)

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