EMPIAR-10330
3.2 Å Single-Particle Cryo-EM Reconstruction of 49 kDa Membrane-Bound PfCRT Complexed with Fab [multiple data sets in MRCS and MRC formats]
Publication:

Structure and drug resistance of the Plasmodium falciparum transporter PfCRT

Kim JK, Tan YZT, Wicht KJW, Erramilli SKE, Dhingra SKD, Okombo JO, Vendome JV, Hagenah LMH, Giacometti SIG, Warren ALW, Nosol KN, Roepe PDR, Potter CSP, Carragher BC, Kossiakoff AAK, Quick MQ, Fidock DAF, Mancia FM

Nature 576 (2019) 315-320

PMID: 31776516

Related EMDB entry:
Deposited:
2019-10-06
Released:
2019-12-19
Last modified:
2020-01-29
Imageset size:
830.42 GB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
  • micrographs - single frame
  • picked particles - single frame - processed
1. Unaligned and compressed multi-frame micrographs
Category:
micrographs - multiframe
Image format:
MRCS
No. of images or tilt series:
3383
Image size:
(3838, 3710)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(0.5175, 0.5175)
Details:
Data collection was performed using a dose of ~91.56 e-/Å2 across 80 frames (75 msec per frame) at a dose rate of ~4.0 e–/pix/sec, using a set defocus range of -1.2 μm to -1.8 μm. Raw frames were compressed using pbzip2. Gain reference image to use is 18jun08i_08213559_01_3710x3838_norm_0_mod.mrc. Magnification anisotropy is present (Distortion angle of 172.7 degrees, major scale of 1.007 and minor scale of 0.993). The script to correct for magnification anisotropy (mag_distort_correct4.cs is attached, and it requires the mag_distortion_correct software available from the Grigorieff lab website.
Files:
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2. Aligned and magnification anisotropy corrected micrographs
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
3297
Image size:
(3838, 3710)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.5175, 0.5175)
Details:
Micrographs aligned using MotionCor2, magnification anisotropy corrected. Alignment was done initially using 3x3 patches (Patch3_micrographs). Micrographs that had estimated resolution at 0.5 confidence level of worse than 10 Å were removed, resulting in 3,297 micrographs. Patch2_micrographs were used later during refinement - hence only a subset of micrographs were aligned using 2x2 patches (Patch2_micrographs).
Files:
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3. Final Particle Stack with Refined Euler Angles and Shifts
Category:
picked particles - single frame - processed
Image format:
MRC
No. of images or tilt series:
16905
Image size:
(300, 300)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.035, 1.035)
Details:
Final particle stack, with Relion .star file containing the refined Euler angles and shifts.
Files:
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Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)
Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)
Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)
Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)