EMPIAR-10269 - Single particle reconstruction of 52 kDa apo-state streptavidin at 3.3 Angstrom resolution [1448 multi-frame micrographs composed of 32 frames each in MRC format]

EMPIAR-10269

Single particle reconstruction of 52 kDa apo-state streptavidin at 3.3 Angstrom resolution [1448 multi-frame micrographs composed of 32 frames each in MRC format]

Publication:

Single particle cryo-EM reconstruction of 52 kDa streptavidin at 3.2 Angstrom resolution

Fan X, Wang J, Zhang X, Yang Z, Zhang JC, Zhao L, Peng HL, Lei J, Wang HW

Nature communications 10 (2019)

PMID: 31160591

DOI: 10.1038/s41467-019-10368-w

Related EMDB entry:

EMD-0690

Deposited:

2019-03-05

Released:

2019-06-07

Last modified:

2019-06-07

Imageset size:

2.40 TB

Imageset DOI:

10.6019/EMPIAR-10269

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Uncorrected apo-state streptavidin movie stacks, binning 2 from super-resolution stacks.

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

1448

Image size:

(3838, 3710)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.5265, 0.5265)

Details:

particle_coordinates/run_data_23991_final_refine.star
23991 particles for final 3D reconstruction.

particle_coordinates/After2D_split/After2D_onG_134606_particles.star
After 2D classification, 134,606 particles on selected 749 images were assigned to subset A (GWI) according to the special pattern.

particle_coordinates/After2D_split/After2D_onICE_77499_particles.star
After 2D classification, 77,499 particles on selected 749 images were assigned to subset B (AWI) according to the special pattern.

particle_coordinates/After3D_split/after3d_onG_35676.star
After 1st round 3D classification, 35,676 particles on selected 749 images were assigned to subset A (GWI) according to the special pattern.

particle_coordinates/After3D_split/after3d_onICE_8650.star
After 1st round 3D classification, 8,650 particles on selected 749 images were assigned to subset B (AWI) according to the special pattern.

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