EMPIAR-10268 - Separating distinct macromolecular assemblies from cryo-EM images [2423 micrographs in MRC format]

EMPIAR-10268

Separating distinct macromolecular assemblies from cryo-EM images [2423 micrographs in MRC format]

Publication:

Separating distinct structures of multiple macromolecular assemblies from cryo-EM projections

Verbeke EJ, Zhou Y, Horton AP, Mallam AL, Taylor DW, Marcotte EM

Journal of structural biology 209 (2019)

PMID: 31726096

DOI: 10.1016/j.jsb.2019.107416

Related EMDB entry:

EMD-20109 EMD-20112 EMD-20110 EMD-20111

Deposited:

2019-04-16

Released:

2020-07-14

Last modified:

2020-07-14

Imageset size:

128.53 GB

Imageset DOI:

10.6019/EMPIAR-10268

Experimental metadata:

Download xml json

Contains:

micrographs - single frame

Image Sets

1. Drift-corrected and dose-weighted average micographs of a mixture containing 40S, 60S, 80S and apoferritin

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

2423

Image size:

(3710, 3838)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.1, 1.1)

Details:

These micrographs are the average after alignment and dose-weighting of 20 raw movie frames (accumulating 40 electron per squared Angstrom in a 6 second exposure).

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Citations

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Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

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Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

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