EMPIAR-10250 - Human methemoglobin movies obtained using Talos Arctica operating at 200 kV equipped with a K2 [1665 multi-frame micrographs composed of 44 frames each in MRC format]

EMPIAR-10250

Human methemoglobin movies obtained using Talos Arctica operating at 200 kV equipped with a K2 [1665 multi-frame micrographs composed of 44 frames each in MRC format]

Publication:

High-resolution structure determination of sub-100 kilodalton complexes using conventional cryo-EM

Herzik Jr MA, Wu M, Lander GC

bioRxiv (---)

DOI: 10.1101/489898

Related EMDB entry:

EMD-0407 EMD-0408

Deposited:

2018-12-19

Released:

2019-03-18

Last modified:

2019-03-18

Imageset size:

2.09 TB

Imageset DOI:

10.6019/EMPIAR-10250

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Raw, unaligned movie stacks of human methemoglobin acquired on a Talos Arctica using a K2 direct electron detector

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

1665

Image size:

(3710, 3838)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(0.5585, 0.5585)

Details:

Raw, unaligned counting mode frame stacks (44 frames/movie) of human methemoglobin in vitreous ice imaged on a Talos Arctica using a K2 direct electron detector. Two separate data collections that were combined are provided. Images used for corrections are also included (require flip along Y axis).

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Citations

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PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

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Overcoming Preferred Orientation in Cryo-EM With Ultrasonic Excitation During Vitrification

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