EMPIAR-10249 - Horse liver alcohol dehydrogenase movies obtained using Talos Arctica operating at 200 kV equipped with a K2 [1153 multi-frame micrographs composed of 44 frames each in MRC format]

EMPIAR-10249

Horse liver alcohol dehydrogenase movies obtained using Talos Arctica operating at 200 kV equipped with a K2 [1153 multi-frame micrographs composed of 44 frames each in MRC format]

Publication:

High-resolution structure determination of sub-100 kilodalton complexes using conventional cryo-EM

Herzik Jr MA, Wu M, Lander GC

bioRxiv (2018)

DOI: 10.1101/489898

Related EMDB entry:

EMD-0406

Deposited:

2018-12-10

Released:

2019-03-18

Last modified:

2019-03-18

Imageset size:

146.16 GB

Imageset DOI:

10.6019/EMPIAR-10249

Experimental metadata:

Download xml json

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

MRC movies (*frames.mrc) in this entry were compressed by bzip2 (pbzip2)
by EMPIAR Japan to save space and bandwidth.

Some programs (e.g. RELION >= 4.0.1 and CryoSPARC) can process compressed movies without explicit
decompression. For other programs, movies have to be decompressed beforehand. In this case,
you might want to convert decompressed MRC movies into TIFF to save your local storage.

The original entry contained one temporary file (17aug05a/rawdata/17aug05a_00016hl16_00003edhiii.frames.tif.tmp),
which was removed.

Original size:   1,408,437,840 kilobytes
Compressed size:   153,147,141 kilobytes

Contains:

micrographs - multiframe

Image Sets

1. Raw, unaligned movies of horse liver alcohol dehydrogenase acquired on a Talos Arctica using a K2 direct electron detector

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

1153

Image size:

(3710, 3838)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(0.5585, 0.5585)

Details:

Raw, unaligned counting mode frame stacks (44 frames) of horse liver alcohol dehydrogenase in vitreous ice imaged on a Talos Arctica using a K2 direct electron detector. Two separate data collections that were combined are provided. Images used for corrections are also included (require flip in Y).

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Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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