EMPIAR-10216 - Cryo-EM structure of mouse heavy-chain apoferritin at 1.62 A [1647 multi-frame micrographs composed of 59 frames each in MRC format]

EMPIAR-10216

Cryo-EM structure of mouse heavy-chain apoferritin at 1.62 A [1647 multi-frame micrographs composed of 59 frames each in MRC format]

Publication:

Cryo-Electron Microscopy Methodology: Current Aspects and Future Directions

Danev R, Yanagisawa H, Kikkawa M

Trends in biochemical sciences 44 (2019) 837-848

PMID: 31078399

DOI: 10.1016/j.tibs.2019.04.008

Related EMDB entry:

EMD-9599

Deposited:

2018-10-04

Released:

2018-10-25

Last modified:

2022-01-31

Imageset size:

1.18 TB

Imageset DOI:

10.6019/EMPIAR-10216

Experimental metadata:

Download xml json

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

MRC movies (FoilHole*Fractions.mrc) in this entry were compressed by bzip2 (pbzip2)
by EMPIAR Japan to save space and bandwidth.

Some programs (e.g. RELION >= 4.0.1 and CryoSPARC) can process compressed movies without explicit
decompression. For other programs, movies have to be decompressed beforehand. In this case,
you might want to convert decompressed MRC movies into TIFF to save your local storage.

Original size:   3,184,166,511 kilobytes
Compressed size: 1,267,082,574 kilobytes

Contains:

micrographs - multiframe

Image Sets

1. RAW movies

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

1647

Image size:

(4096, 4096)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(0.517, 0.517)

Details:

- Dataset details are in the "dataset_details.txt" file.
- "particles_data.star" provides information about all particles BEFORE polishing and CTF refinement
- "polished_CTFrefined_particles_data.star" provides information about all particles AFTER polishing and CTF refinement. Note: use this file for the refined defocus information. Particle coordinates may not be accurate

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Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

Structural basis for HIV-1 antagonism of host APOBEC3G via Cullin E3 ligase.

Ito F, Alvarez-Cabrera AL, Liu S, Yang H, Shiriaeva A, Zhou ZH, Chen XS. (2023)

The 2023 Nucleic Acids Research Database Issue and the online molecular biology database collection.

Rigden DJ, Fernández XM. (2023)

EMPIAR: the Electron Microscopy Public Image Archive.

Iudin A, Korir PK, Somasundharam S, Weyand S, Cattavitello C, Fonseca N, Salih O, Kleywegt GJ, Patwardhan A. (2023)

CLEMSite, a software for automated phenotypic screens using light microscopy and FIB-SEM.

Serra Lleti JM, Steyer AM, Schieber NL, Neumann B, Tischer C, Hilsenstein V, Holtstrom M, Unrau D, Kirmse R, Lucocq JM, Pepperkok R, Schwab Y. (2023)

Structure of a RecT/Redβ family recombinase in complex with a duplex intermediate of DNA annealing.

Caldwell BJ, Norris AS, Karbowski CF, Wiegand AM, Wysocki VH, Bell CE. (2022)

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