EMPIAR-10193 - Calicivirus VP2 forms a portal to mediate endosome escape [13872 micrographs in MRC format]

EMPIAR-10193

Calicivirus VP2 forms a portal to mediate endosome escape [13872 micrographs in MRC format]

Publication:

Calicivirus VP2 forms a portal-like assembly following receptor engagement

Conley MJ, McElwee M, Azmi LB, Gabrielsen M, Byron O, Goodfellow IG, Bhella D

Nature (2019)

PMID: 30626974

DOI: 10.1038/s41586-018-0852-1

Related EMDB entry:

EMD-0056

Deposited:

2018-06-12

Released:

2019-01-18

Last modified:

2019-01-18

Imageset size:

930.95 GB

Imageset DOI:

10.6019/EMPIAR-10193

Experimental metadata:

Download xml json

Contains:

micrographs - single frame

Image Sets

1. Motion corrected micrographs of feline calicivirus strain F9 decorated with feline junctional adhesion molecule A

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

13872

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.065, 1.065)

Details:

Data recorded on a Thermo Fisher Titan Krios at Astbury BioStructure Laboratory, using a Falcon III camera.
Nominal magnification: 75000X
Exposure time: 1.5 seconds
Fractions: 50
Dose rate 1.26 e/angstrom/fraction
Motion correction performed using motioncor2 with dose weighting.

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