EMPIAR-10188 - T20S proteasome single particle [586 micrographs in MRC format]

EMPIAR-10188

T20S proteasome single particle [586 micrographs in MRC format]

Publication:

Routine single particle CryoEM sample and grid characterization by tomography

Noble AJ, Dandey VP, Wei H, Brasch J, Chase J, Acharya P, Tan YZ, Zhang Z, Kim LY, Scapin G, Rapp M, Eng ET, Rice WJ, Cheng A, Negro CJ, Shapiro L, Kwong PD, Jeruzalmi D, des Georges A, Potter CS, Carragher B

eLife 7 (2018)

PMID: 29809143

DOI: 10.7554/elife.34257

Related EMDB entry:

EMD-7153

Deposited:

2018-05-10

Released:

2018-05-14

Last modified:

2022-03-22

Imageset size:

124.69 GB

Imageset DOI:

10.6019/EMPIAR-10188

Experimental metadata:

Download xml json

Contains:

micrographs - single frame

Image Sets

1. Unaligned, frame aligned, and frame aligned+dose weighted micrographs

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

586

Image size:

(3710, 3838)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.073, 1.073)

Details:

Full Leginon data collection session. Includes all magnification images. Three images are included for each exposure area: enn (unaligned frame sum), enn-a (aligned frame sum), and enn-a-DW (aligned frame sum with dose weighting). Frame alignment and dose weighting was performed with MotionCor2.

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Citations

High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles

Kim K, Li H, Clarke OB. (2025)

CLEM-Reg: an automated point cloud-based registration algorithm for volume correlative light and electron microscopy.

Krentzel D, Elphick M, Domart MC, Peddie CJ, Laine RF, Shand C, Henriques R, Collinson LM, Jones ML. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

Laser flash melting cryo-EM samples to overcome preferred orientation.

Straub MS, Harder OF, Mowry NJ, Barrass SV, Hruby J, Drabbels M, Lorenz UJ. (2025)

In situ structure determination of conformationally flexible targets using nextPYP.

Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)

CryoPROS: Correcting misalignment caused by preferred orientation using AI-generated auxiliary particles.

Zhang H, Zheng D, Wu Q, Yan N, Peng H, Hu Q, Peng Y, Yan Z, Shi Z, Bao C, Hu M. (2025)

Few-shot learning for non-vitrified ice segmentation.

Vivas-Lago A, Castaño-Díez D. (2025)

Automated removal of corrupted tilts in cryo-electron tomography.

Majtner T, Turoňová B. (2025)

Probabilistic single-particle cryo-EM ab initio 3D reconstruction in SIMPLE.

Van CTS, Reboul CF, Caesar JJE, Meana-Pañeda R, Lountos GT, Deme JC, Bryant OJ, Johnson S, Piczak CT, Valkov E, Lea SM, Elmlund H. (2025)

Reanalysis of cryo-EM data reveals ALK-cytokine assemblies with both 2:1 and 2:2 stoichiometries.

Felix J, De Munck S, Bazan JF, Savvides SN. (2025)

See all citations

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