EMPIAR-10176
Single particle cryo-EM dataset of the flexible and variable oligomeric state complex AP-1:Arf1:tetherin-HIV-Nef [stack of 209816 particles in MRCS format]
Publication:

HIV-1 Nefs Are Cargo-Sensitive AP-1 Trimerization Switches in Tetherin Downregulation

Morris KL, Buffalo CZ, Stürzel CM, Heusinger E, Kirchhoff F, Ren X, Hurley JH

Cell 174 (2018) 659-671.e14

PMID: 30053425

Related EMDB entry:
Deposited:
2018-03-06
Released:
2018-07-27
Last modified:
2018-07-27
Imageset size:
115.37 GB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • picked particles - multiframe - unprocessed
1. Dose weighted particle stack of AP1:Arf1:tetherin-HIV-Nef
Category:
picked particles - multiframe - unprocessed
Image format:
MRCS
No. of images or tilt series:
209816
Image size:
(384, 384)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.067, 1.067)
Details:
Picked particles after 2D class averaging in Relion to clean the dataset. 209,816 particles represent the AP1:Arf1:tetherin-Nef dataset before 3D classification of the multiple oligomeric and flexible states of the complex.
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