EMPIAR-10169
CryoET of apoferritin single particle with spot-to-plunge time of 100ms [multiple data sets in MRC and DYNAMO TBL AND OMD FILES formats]
Publication:

Reducing effects of particle adsorption to the air-water interface in cryoEM

Noble AJ, Wei H, Dandey VP, Zhang Z, Potter CS, Carragher B

BioRxiv (---)

Related EMDB entry:
Deposited:
2018-03-23
Released:
2018-04-05
Last modified:
2018-04-05
Imageset size:
119.33 GB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
  • micrographs - single frame
  • particle picking
  • tilt series
1. K2 tilt-series frames
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
15
Image size:
(3710, 3838)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(2.16423, 2.16423)
Details:
~8 e-/px/s dose rate on the camera
Gain and dark references included
Includes tilt-series from the same session (apoferritin at 400ms spot-to-plunge time and insulin-bound insulin receptor at 600ms spot-to-plunge time)
Files:
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2. Whole-frame aligned tilt images along with all other magnification images from the collection
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
15
Image size:
(3710, 3838)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(2.16423, 2.16423)
Details:
Whole-frame aligned tilt images using Motioncor2.
Includes tilt-series from the same session (apoferritin at 400ms spot-to-plunge time and insulin-bound insulin receptor at 600ms spot-to-plunge time).
Files:
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3. Manual particle picking in Dynamo
Category:
particle picking
Image format:
DYNAMO TBL AND OMD FILES
No. of images or tilt series:
1
Image size:
(None, None)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(None, None)
Details:
Binned by 4 manual particle picking of the series0001 tomogram using Dynamo. Manual picking was performed to accurately distinguish between particles adsorbed to the air-water interfaces, non-adsorbed particles, and particles over or adsorbed to carbon.
Files:
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4. Appion-Protomo tilt-series alignments
Category:
tilt series
Image format:
MRC
No. of images or tilt series:
6
Image size:
(3710, 3838)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(2.16423, 2.16423)
Details:
Includes a full tilt-series alignment run for all 100ms spot-to-plunge time apoferritin tilt-series.
To view properly, navigate to the protomo_alignments folder in Appion-Protomo.
The export/ directory contains SerialEM-formatted unaligned tilt-series stacks, mdoc files, and tilt files. This allows for easy upload to Appion-Protomo.
Files:
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Files:
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