The micrographs were the drift-corrected averages of 38 raw movie frames. Each original movie was recored in 40 frames accumulating 53 electron per squared angstrom in 10 second exposure, with the first two frame not being used after drift correction for the averaged micrographs.
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The picked particles were binned twice to 140x140 box size and a pixel size of 1.52 angstrom.
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