EMPIAR-10158 - Cryo electron tomography of immotile sea urchin sperm flagella [27 tilt series in MRC format]

EMPIAR-10158

Cryo electron tomography of immotile sea urchin sperm flagella [27 tilt series in MRC format]

Publication:

Asymmetric distribution and spatial switching of dynein activity generates ciliary motility

Lin J, Nicastro D

Science (New York, N.Y.) 360 (2018)

PMID: 29700238

DOI: 10.1126/science.aar1968

Related EMDB entry:

EMD-8837 EMD-8835

Deposited:

2018-02-16

Released:

2018-04-27

Last modified:

2021-05-28

Imageset size:

13.77 GB

Imageset DOI:

10.6019/EMPIAR-10158

Experimental metadata:

Download xml json

Contains:

tilt series

Image Sets

1. Tilt series of immotile sea urchin sperm flagella

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

Image size:

(2048, 2048)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(9.856, 9.856)

Details:

These 27 tilt series were collected from two different types of control samples using a FEI Tecnai F30 electron microscope and were control data of EMPIAR-10157. A sub-tomogram average derived from the first 20 tilt series (S1-S20) of EHNA inhibited immotile sea urchin sperm flagella has been deposited in EMDB under ID code EMD-8835; A sub-tomogram average derived from the last 7 tilt series (S21-S27) of the other control sample (Triton X-100 demembranated sea urchin flagella) has been deposited in EMDB under ID code EMD-8837.

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